A H2O2-forming peroxidase rather than a NAD(P)H-dependent O2•- synthase may be the major player in cell death responses controlled by the Pto-Fen complex following fenthion treatment

Functional Plant Biology : FPB
Margherita G De BiasiGian Piero Soressi

Abstract

Four tomato (Lycopersicon esculentum Mill.) near-isogenic lines were treated by foliar spraying with the insecticide fenthion. Two, Riogrande and Rimone, differed from each other only for the presence in the latter of the Fen gene, conferring propensity to develop foliar symptoms upon exposure to fenthion. The other two, namely RC332 and RC131, were the transgenic versions of Riogrande and Rimone, respectively, harbouring the Gox gene encoding for glucose oxidase of Aspergillus niger. The production of H2O2 as well as the activities of H+-ATPase, NAD(P)H-dependent superoxide synthase, catalase, peroxidase, and Cu,Zn-superoxide dismutase were evaluated in the foliar tissues up to 24 h after exposure to fenthion. The Fen gene conferred sensitivity to fenthion, regardless of the expression of a Gox transgene. A prolonged accumulation of H2O2 was observed in the leaves of Rimone and of RC131, which was instead transient in Riogrande and in RC332. In all the tomato lines, exposure to fenthion induced rapid but transient changes in the activities of most enzymes. The only exception was peroxidase activity in the leaves of Rimone and of RC131, which steadily increased until the end of the sampling period. It is suggested that the sens...Continue Reading

Citations

Jan 7, 2010·Protoplasma·María Florencia IannoneMaría Patricia Benavides
Sep 21, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Luciane Belmonte PereiraMaria R C Schetinger
Nov 22, 2011·Biological Trace Element Research·María Florencia IannoneMaría Patricia Benavides
Oct 11, 2017·Environmental Science and Pollution Research International·Margherita G De BiasiMaurizio Badiani
Mar 1, 2006·Functional Plant Biology : FPB·Daymi CamejoFrancisca Sevilla

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