A hamster-derived West Nile virus strain is highly attenuated and induces a differential proinflammatory cytokine response in two murine cell lines.

Virus Research
Vandana SaxenaTian Wang

Abstract

Increasing evidence suggests that West Nile virus (WNV) induces a persistent infection in some humans and animals. Here, we characterized infection of mouse macrophage and kidney epithelial cell lines with a strain of WNV (H8912), cultured from urine of a persistently infected hamster. WNV H8912 had a reduced replication rate, concurrent with a lower interferon (IFN)-β gene expression in both cell types compared to its parent strain - WNV NY99. In WNV H8912-infected macrophages, we observed higher interleukin (IL)-6 and tumor necrosis factor (TNF)-α expression and more nuclear factor kappa B (NF-κB) activation than in cells infected with WNV NY99. In contrast, there were reduced levels of TNF-α and IL-6 expression, as well as less NF-κB activation following WNV H8912 infection in the kidney epithelial cells compared to WNV NY99. Overall, our results demonstrate that the WNV isolate obtained from hamster urine is an attenuated virus and induces a differential proinflammatory cytokine response in mouse macrophage and kidney epithelial cell lines.

References

Jan 1, 1983·Archives of Virology·V V PogodinaN M Ralph
Jul 1, 1983·The Journal of Experimental Medicine·M J CardosaS Gordon
Oct 3, 2002·Nature Reviews. Immunology·Qiutang Li, Inder M Verma
Jan 16, 2003·Immunology Letters·Shizuo Akira, Hiroaki Hemmi
Dec 10, 2003·The Journal of Experimental Medicine·Michael S DiamondMichael Engle
May 6, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kadiyala V RavindraR Brian Stevens
Jul 16, 2004·Journal of Virology·Bimmi Shrestha, Michael S Diamond
Sep 18, 2004·European Journal of Immunology·Francesco DieliAlfredo Salerno
Sep 30, 2004·Nature Immunology·Akiko Iwasaki, Ruslan Medzhitov
Feb 1, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Robert G PennSherif R Zaki
Aug 17, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Paul J CarsonRoss D Crosby
Oct 13, 2006·Journal of Virology·Elizabeth M Sitati, Michael S Diamond
Jun 15, 2007·European Journal of Immunology·James D BrienJanko Nikolich-Zugich
Jul 25, 2007·Annual Review of Entomology·Laura D KramerGregory D Ebel
Sep 21, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Duane J Gubler
Jun 20, 2008·Journal of Virology·Jason R WilsonFrank Scholle
Aug 30, 2008·The Journal of Infectious Diseases·Michael P BuschSally Caglioti
Nov 15, 2008·The Journal of General Virology·Xiaoyan WuShu-Yuan Xiao
Jun 11, 2009·Journal of Clinical and Experimental Neuropsychology·Joseph R SadekKathleen Y Haaland
Dec 8, 2009·The Journal of Infectious Diseases·Kristy MurraySusan Fisher-Hoch
Nov 3, 2010·The American Journal of Tropical Medicine and Hygiene·Robert L CookCharles R Rinaldo
Aug 13, 2011·Journal of Medical Virology·Anna PapaTakis Panagiotopoulos

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Citations

Jun 21, 2013·PLoS Neglected Tropical Diseases·Vandana SaxenaTian Wang
May 1, 2013·Expert Review of Anti-infective Therapy·Luisa BarzonGiorgio Palù
Aug 10, 2019·Microbiology Resource Announcements·Christina JacobBernadette J Connors
Apr 24, 2019·International Journal of Molecular Sciences·Shannon E RoncaKristy O Murray

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