A hard microflow cytometer using groove-generated sheath flow for multiplexed bead and cell assays.

Analytical and Bioanalytical Chemistry
Abel L ThangawngFrances S Ligler

Abstract

With a view toward developing a rugged microflow cytometer, a sheath flow system was micromachined in hard plastic (polymethylmethacrylate) for analysis of particles and cells using optical detection. Six optical fibers were incorporated into the interrogation region of the chip, in which hydrodynamic focusing narrowed the core stream to ~35 μm × 40 μm. The use of a relatively large channel at the inlet as well as in the interrogation region (375 μm × 125 μm) successfully minimized the risk of clogging. The device could withstand pressures greater than 100 psi without leaking. Assays using both coded microparticles and cells were demonstrated using the microflow cytometer. Multiplexed immunoassays detected nine different bacteria and toxins using a single mixture of coded microspheres. A549 cancer cells processed with locked nucleic acid probes were evaluated using fluorescence in situ hybridization.

References

Nov 1, 2003·Analytical Chemistry·Maxine A McClainJ Michael Ramsey
Mar 3, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Howard M Shapiro
Apr 5, 2007·Nature Protocols·Gabriela M BaerlocherPeter M Lansdorp
Jan 30, 2008·Analytical and Bioanalytical Chemistry·Daniel A AteyaFrances S Ligler
Jun 28, 2008·Lab on a Chip·Peter B HowellFrances S Ligler
Jun 6, 2009·Analytical Chemistry·Jason S KimFrances S Ligler
Jun 18, 2009·Lab on a Chip·Joel P GoldenFrances S Ligler
Oct 1, 1999·Analytical Chemistry·D P SchrumJ M Ramsey

❮ Previous
Next ❯

Citations

Jun 8, 2010·Analytical and Bioanalytical Chemistry·Jason S Kim, Frances S Ligler
Sep 29, 2011·Analytical and Bioanalytical Chemistry·Joel P GoldenFrances S Ligler
Jul 16, 2011·Analytical Chemistry·Jeffrey S EricksonFrances S Ligler
Feb 22, 2012·Lab on a Chip·Jasenka VerbargFrances S Ligler
Oct 10, 2012·Lab on a Chip·Joerg MartiniPeter Kiesel
Nov 1, 2012·Sensors·Yekbun Adiguzel, Haluk Kulah
Jul 30, 2011·Trends in Biotechnology·David S BoyleXuanhong Cheng
May 28, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Marcin FrankowskiJörg Neukammer
Dec 30, 2014·Biomicrofluidics·Hai JiangDongqing Li
Feb 15, 2013·Scientific Reports·C LiberaleI Cristiani
Apr 11, 2013·Sensors·Marcin FrankowskiJörg Neukammer
Feb 4, 2014·Lab on a Chip·Menake E Piyasena, Steven W Graves
Jun 27, 2014·Lab on a Chip·Daniel SpencerHywel Morgan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.