A harpin binding site in tobacco plasma membranes mediates activation of the pathogenesis-related gene HIN1 independent of extracellular calcium but dependent on mitogen-activated protein kinase activity

The Plant Cell
Justin LeeT Nürnberger

Abstract

Harpin from the bean halo-blight pathogen Pseudomonas syringae pv phaseolicola (harpin(Psph)) elicits the hypersensitive response and the accumulation of pathogenesis-related gene transcripts in the nonhost plant tobacco. Here, we report the characterization of a nonproteinaceous binding site for harpin(Psph) in tobacco plasma membranes, which is assumed to mediate the activation of plant defense responses in a receptor-like manner. Binding of 125I-harpin(Psph) to tobacco microsomal membranes (dissociation constant = 425 nM) and protoplasts (dissociation constant = 380 nM) was specific, reversible, and saturable. A close correlation was found between the abilities of harpin(Psph) fragments to elicit the transcript accumulation of the pathogenesis-related tobacco gene HIN1 and to compete for binding of 125I-harpin(Psph) to its binding site. Another elicitor of the hypersensitive response and HIN1 induction in tobacco, the Phytophthora megasperma-derived beta-elicitin beta-megaspermin, failed to bind to the putative harpin(Psph) receptor. In contrast to activation by beta-megaspermin, harpin(Psph)-induced activation of the 48-kD salicylic acid-responsive mitogen-activated protein kinase (MAPK) and HIN1 transcript accumulation wer...Continue Reading

References

Oct 1, 1991·Current Opinion in Cell Biology·C E Turner, K Burridge
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·T NürnbergerD Scheel
Oct 1, 1996·The Plant Journal : for Cell and Molecular Biology·S GopalanS Y He
Feb 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·N UmemotoI Ishida
May 1, 1997·The Plant Cell·S Zhang, D F Klessig
Jul 1, 1997·Genes & Development·Y YangD F Klessig
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·S Zhang, D F Klessig
Jul 11, 1998·The Journal of Biological Chemistry·M F FavataJ M Trzaskos
Nov 10, 1998·The Plant Journal : for Cell and Molecular Biology·A Lebrun-GarciaA Pugin
Mar 6, 1999·Current Opinion in Plant Biology·D Scheel
Apr 3, 1999·Cellular and Molecular Life Sciences : CMLS·C JonakH Hirt
Aug 25, 1999·Current Opinion in Plant Biology·M Grant, J Mansfield
Nov 27, 1999·The Journal of Biological Chemistry·S BourqueA Lebrun-Garcia
Mar 14, 2000·The Journal of Biological Chemistry·T S NühseT Boller
Aug 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·K ThevissenW F Broekaert
Aug 17, 2000·Plant & Cell Physiology·G FellbrichT Nürnberger
Sep 12, 2000·Plant Physiology·B D Kohorn
Jan 3, 2001·Proceedings of the National Academy of Sciences of the United States of America·J LeeT Nürnberger

❮ Previous
Next ❯

Citations

Apr 23, 2003·Microbes and Infection·Qiaoling JinSheng-Yang He
Aug 10, 2001·Trends in Plant Science·T Nürnberger, D Scheel
Nov 10, 2001·Trends in Plant Science·S Zhang, D F Klessig
Oct 13, 2001·Current Opinion in Plant Biology·T Romeis
Aug 17, 2002·Current Opinion in Plant Biology·Claudia JonakHeribert Hirt
Jul 23, 2003·Current Opinion in Plant Biology·Hans Thordal-Christensen
Jan 1, 2003·Molecular Plant Pathology·Marcos MontesanoE Tapio Palva
Nov 2, 2002·The Plant Journal : for Cell and Molecular Biology·Guido FellbrichThorsten Nürnberger
Jun 13, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christian OttmannClaudia Oecking
Oct 11, 2002·The EMBO Journal·Daniela Büttner, Ulla Bonas
Jan 10, 2012·Journal of Experimental Botany·Ahmed IsmailPeter Nick
Jun 12, 2013·Molecular Plant-microbe Interactions : MPMI·Min-Seon ChoiChang-Sik Oh
Jan 28, 2005·Molecular Plant-microbe Interactions : MPMI·Chun-Mei LiSuvi Taira
Mar 31, 2006·Molecular Plant-microbe Interactions : MPMI·Takashi FujikawaShinji Tsuyumu
Jul 15, 2006·Molecular Plant-microbe Interactions : MPMI·Angela Garcia-BruggerAlain Pugin
Oct 10, 2008·Molecular Plant-microbe Interactions : MPMI·Marco LoehrerUlrich Schaffrath
Feb 9, 2008·Molecular Plant-microbe Interactions : MPMI·Viola A ManningLynda M Ciuffetti
Dec 17, 2002·Molecular Plant-microbe Interactions : MPMI·Nienke WesterinkMatthieu H A J Joosten
Jun 11, 2003·Molecular Plant-microbe Interactions : MPMI·Benoît PoinssotAlain Pugin
Dec 16, 2004·Molecular Plant-microbe Interactions : MPMI·Laura MattinenMinna Pirhonen
May 12, 2010·Annual Review of Phytopathology·Anastasia P TampakakiNickolas J Panopoulos
Jun 4, 2013·Annual Review of Phytopathology·Muthappa Senthil-Kumar, Kirankumar S Mysore
May 3, 2005·Annual Review of Plant Biology·Nicolas BouchéHillel Fromm
Dec 2, 2011·Journal of Biomedicine & Biotechnology·Tamara ZimaroJorgelina Ottado

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.