A heat-sensitive Arabidopsis thaliana kinase substitutes for human p70s6k function in vivo.

Molecular and Cellular Biology
F TurckF Nagy

Abstract

In mammalian cells, mitogen-induced phosphorylation of ribosomal protein S6 by p70s6k has been implicated in the selective translational upregulation of 5'TOP mRNAs. We demonstrate here that the homologous Arabidopsis thaliana protein, AtS6k2, ectopically expressed in human 293 cells or isolated from plant cells, phosphorylates specifically mammalian and plant S6 at 25 degrees C but not at 37 degrees C. When Arabidopsis suspension culture cells are shifted from 25 to 37 degrees C, the kinase becomes rapidly inactivated, consistent with the observation that heat shock abrogates S6 phosphorylation in plants. Treatment with potato acid phosphatase reduced the specific activity of immunoprecipitated AtS6k2 threefold, an effect which was blocked in the presence of 4-nitrophenyl phosphate. In quiescent mammalian cells, AtS6k2 is activated by serum stimulation, a response which is abolished by the fungal metabolite wortmannin but is resistant to rapamycin. Treatment of mammalian cells with rapamycin abolishes in vivo S6 phosphorylation by p70s6k; however, ectopic expression of AtS6k2 rescues the rapamycin block. Collectively, the data demonstrate that AtS6k2 is the functional plant homolog of mammalian p70s6k and identify a new signal...Continue Reading

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Citations

Aug 21, 2012·Current Opinion in Plant Biology·Bart Rymen, Keiko Sugimoto
Feb 26, 2016·Annual Review of Plant Biology·Thomas DobrenelChristian Meyer
Aug 16, 2008·International Review of Cell and Molecular Biology·Oded Meyuhas
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May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·Benoît MenandChristophe Robaglia
Jul 26, 2005·Current Opinion in Plant Biology·Roman Ulm, Ferenc Nagy
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Apr 12, 2005·Plant Molecular Biology·Patrick GiavaliscoPaola Fucini
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Oct 17, 2006·Oncogene·J H Reiling, D M Sabatini
Aug 8, 2008·BMC Plant Biology·Jason M AbercrombieC Neal Stewart
Jul 18, 2020·FEBS Letters·Shivani UpadhyayaBasuthkar Jagadeeshwar Rao
Aug 8, 2015·The Biochemical Journal·Daniel RexinBruce Veit
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Jun 16, 2005·Plant Physiology·María Eugenia ZanettiJulia Bailey-Serres
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Apr 6, 2004·Plant Physiology·Franziska TurckFerenc Nagy
Jun 7, 2006·Cell Metabolism·Sung Hee UmGeorge Thomas
May 29, 2008·Biochemical and Biophysical Research Communications·Jheralyn MartinJoseph Gera

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