A helix-turn motif in the C-terminal domain of histone H1

Protein Science : a Publication of the Protein Society
R VilaPedro Suau

Abstract

The structural study of peptides belonging to the terminal domains of histone H1 can be considered as a step toward the understanding of the function of H1 in chromatin. The conformational properties of the peptide Ac-EPKRSVAFKKTKKEVKKVATPKK (CH-1), which belongs to the C-terminal domain of histone H1(o) (residues 99-121) and is adjacent to the central globular domain of the protein, were examined by means of 1H-NMR and circular dichroism. In aqueous solution, CH-1 behaved as a mainly unstructured peptide, although turn-like conformations in rapid equilibrium with the unfolded state could be present. Addition of trifluoroethanol resulted in a substantial increase of the helical content. The helical limits, as indicated by (i,i + 3) nuclear Overhauser effect (NOE) cross correlations and significant up-field conformational shifts of the C(alpha) protons, span from Pro100 to Val116, with Glu99 and Ala117 as N- and C-caps. A structure calculation performed on the basis of distance constraints derived from NOE cross peaks in 90% trifluoroethanol confirmed the helical structure of this region. The helical region has a marked amphipathic character, due to the location of all positively charged residues on one face of the helix and all...Continue Reading

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Citations

Jun 10, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Andrzej Kowalski, Jan Pałyga
Mar 16, 2001·Current Opinion in Genetics & Development·J J Hayes, J C Hansen
Aug 14, 2008·Journal of Proteome Research·Kristie L RoseJuan Ausió
Mar 24, 2010·Cell Research·Steven J McBryantJeffrey C Hansen
Jan 16, 2002·Protein Science : a Publication of the Protein Society·Roger VilaPedro Suau
Oct 19, 2012·Cell Biology International·Andrzej Kowalski, Jan Pałyga
Feb 24, 2009·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Nikhil RaghuramMichael J Hendzel
Aug 29, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Ryan HeitMichael J Hendzel
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Aug 5, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Xu Lu, Jeffrey C Hansen
Mar 8, 2006·The FEBS Journal·Ewa Górnicka-MichalskaKatarzyna Cywa-Benko
Feb 6, 2013·Organic & Biomolecular Chemistry·María Isabel García-ArandaMaría Jesús Pérez de Vega
Oct 5, 2001·The Journal of Biological Chemistry·R VilaP Suau
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Sep 25, 2003·Cell·Akimitsu KonishiYoshihide Tsujimoto
Oct 1, 2008·Journal of Molecular Biology·Hideyuki TakeshimaShoji Tajima

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