PMID: 11607568Aug 1, 1995Paper

A heme-protein-based oxygen-sensing mechanism controls the expression and suppression of multiple proteins in anoxia-tolerant turtle hepatocytes

Proceedings of the National Academy of Sciences of the United States of America
S C Land, P W Hochachka

Abstract

The O2 sensitivity of protein expression was assessed in hepatocytes from the western painted turtle. Anoxic cells consistently expressed proteins of 83.0, 70.4, 42.5, 35.3, and 16.1 kDa and suppressed proteins of 63.7, 48.2, 36.9, 29.5, and 17.7 kDa. Except for the 70.4-kDa protein, this pattern was absent during aerobic incubation with 2 mM NaCN, suggesting a specific requirement for O2. Aerobic incubation with Co2+ or Ni2+ increased expression of the 42.5-, 35.3-, and 16.1-kDa protein bands which was diminished with the heme synthesis inhibitor 4,6-dioxoheptanoic acid. Proteins suppressed in anoxia were also suppressed during aerobic incubation with Co2+ or Ni2+ but this was not relieved by 4,6-dioxoheptanoic acid. The anoxia- and Co2+/Ni2+-induced expression of the 42.5-, 35.3-, and 16.1-kDa protein bands was antagonized by 10% CO; however, with the exception of the 17.7-kDa protein, this was not found for any of the O2- or Co2+/Ni2+-suppressed proteins. Anoxia-induced proteins were compared with proteins expressed during heat shock. Heat shock proteins appeared at 90.2, 74.8, 63.4, 25, and 15.5 kDa and were of distinct molecular masses compared with the anoxia-induced proteins. These results suggest that O2-sensing mechani...Continue Reading

Citations

Sep 1, 1997·Comparative Biochemistry and Physiology. Part A, Physiology·P W HochachkaL T Buck
Nov 3, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P W Hochachka, P L Lutz
May 12, 1998·Molecular Medicine Today·J M Gleadle, P J Ratcliffe
Sep 25, 2004·American Journal of Respiratory Cell and Molecular Biology·Kristin HeerleinHeimo Mairbäurl
Nov 17, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Patricia M Schulte
Apr 5, 2018·The Journal of Experimental Biology·Jacob B CampbellJon F Harrison
Aug 1, 1997·The American Journal of Physiology·T RoigJ Bermúdez
Jul 17, 1999·The American Journal of Physiology·J W Hicks, T Wang
Apr 1, 1996·Redox Report : Communications in Free Radical Research·G L Wang, G L Semenza
Apr 27, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M GuppyM D Brand
Sep 15, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·B Platzack, J W Hicks
Nov 22, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·P N MutP J Schwarzbaum
Jan 14, 1999·The American Journal of Physiology·W L RumseyM Erecinska
Sep 17, 2004·The Journal of Experimental Biology·Valeria Ramaglia, Leslie T Buck
Dec 13, 2002·The Journal of Experimental Biology·Michelle Anne ScottLeslie Thomas Buck

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.