A hemoglobin-binding outer membrane protein is involved in virulence expression by Haemophilus ducreyi in an animal model.

Infection and Immunity
M K StevensEric J Hansen

Abstract

Haemophilus ducreyi exhibits a requirement for exogenously supplied heme for aerobic growth in vitro. Nine of ten wild-type isolates of H. ducreyi were shown to contain a readily detectable hemoglobin-binding activity. Spontaneous hemoglobin-binding-negative mutants of two of these wild-type isolates lost the ability to express an outer membrane protein with an apparent molecular mass of approximately 100 kDa. Similarly, the single wild-type isolate that lacked the ability to bind hemoglobin also appeared to lack expression of this same 100-kDa protein. A monoclonal antibody (5A9) to this 100-kDa protein was used to identify a recombinant clone which possessed an H. ducreyi chromosomal fragment containing the gene encoding the 100-kDa protein; this protein was designated hemoglobin utilization protein A (HupA). Nucleotide sequence analysis of the hupA gene revealed that the predicted protein, with a calculated molecular mass of 108 kDa, was similar to TonB-dependent outer membrane proteins of other bacteria. Increasing the concentration of heme in the growth medium resulted in decreased expression of the HupA protein. Mutant analysis was used to prove that the HupA protein was essential for the utilization by H. ducreyi of both...Continue Reading

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Citations

Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·L D CopeE J Hansen
May 16, 2002·Infection and Immunity·Joseph R NikaEric J Hansen
Mar 16, 2002·Infection and Immunity·Stanley M SpinolaRobert S Munson
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Sep 21, 2002·AIDS Patient Care and STDs·D A Lewis
Mar 24, 2005·Infection and Immunity·Catherine Dinitra WhiteChristopher Elkins
Sep 24, 2005·Infection and Immunity·Deborah M B PostRobert S Munson
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Aug 4, 2010·Infection and Immunity·Laura L Anzaldi, Eric P Skaar
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Nov 26, 2003·Infection and Immunity·Stanley M SpinolaEric J Hansen
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Nov 13, 1998·Journal of Bacteriology·C K WardE J Hansen
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