A heparin-binding membrane protein from goat spermatozoa immobilizes spermatozoa in the presence of complement

Fertility and Sterility
Visesato MorT Chatterjee

Abstract

To characterize the major membrane protein of goat spermatozoa. Basic research. Samples collected from local slaughterhouse and study conducted in an academic research environment. Goat epididymal tissue. Goat epididymal tissues were collected immediately after slaughter and the spermatozoa were isolated within 2 hours. Sperm immobilization test was performed with motile spermatozoa at 32 degrees C within 3-4 hours of collection and isolation of the cells. The heparin-binding sperm membrane protein (HBSM) of goat is insensitive to trypsin and its antisera immobilize spermatozoa in presence of complement. Forty-two percent of membrane protein could be extracted with 0.25% (wt/vol) 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) from the isolated sperm membrane. By heparin-affinity chromatography, 46% of the extracted protein was recovered. Positive hybridization with radiolabeled heparin on western transfer confirmed the heparin-binding property of the protein (HBSM). Heparin binding to HBSM is an ionic bondage and can be disrupted by 1 M NaCl, as revealed by 86% recovery of the radiolabeled heparin in trichloroacetic acid-precipitated supernatant of [(125)I] heparin-HBSM conjugate. Heparin-binding sperm membr...Continue Reading

Citations

Jan 16, 2007·Biochemical and Biophysical Research Communications·Visesato Mor, Tapati Chatterjee
Nov 28, 2006·Biochemical and Biophysical Research Communications·Visesato MorTapati Chatterjee
Feb 19, 2013·Cellular Immunology·Sándor SomodiPéter Hajdu

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