A herpes simplex virus type 1 gamma34.5 second-site suppressor mutant that exhibits enhanced growth in cultured glioblastoma cells is severely attenuated in animals

Journal of Virology
Ian MohrY Gluzman

Abstract

We describe here the neurovirulence properties of a herpes simplex virus type 1 gamma34.5 second-site suppressor mutant. gamma34.5 mutants are nonneurovirulent in animals and fail to grow in a variety of cultured cells due to a block at the level of protein synthesis. Extragenic suppressors with restored capacity to replicate in cells that normally do not support the growth of the parental gamma34.5 deletion mutant have been isolated. Although the suppressor virus reacquires the ability to grow in nonpermissive cultured cells, it remains severely attenuated in mice and is indistinguishable from the mutant gamma34.5 parent virus at the doses investigated. Repairing the gamma34.5 mutation in the suppressor mutant restores neurovirulence to wild-type levels. These studies illustrate that (i) the protein synthesis and neurovirulence defects observed in gamma34.5 mutant viruses can be genetically separated by an extragenic mutation at another site in the viral chromosome; (ii) the extragenic suppressor mutation does not affect neurovirulence; and (iii) the attenuated gamma34.5 mutant, which replicates poorly in many cell types, can be modified by genetic selection to generate a nonpathogenic variant that regains the ability to grow ...Continue Reading

References

Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·R ChambersR J Whitley
Jan 1, 1994·Journal of Neuro-oncology·M G KaplittR G Blasberg
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·S S AndreanskyR J Whitley
Feb 17, 1999·The Journal of Experimental Medicine·D A LeibH W Virgin
Feb 2, 2000·Reviews in Medical Virology·J M MarkertR J Whitley
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·J N ParkerJ M Markert
Apr 5, 2000·The Journal of Clinical Investigation·R L Martuza
May 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·D A LeibH W Virgin

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Citations

Feb 9, 2011·Immunology and Cell Biology·Daniëlle HorstEmmanuel J H J Wiertz
Oct 19, 2005·Gene Therapy·R ArgnaniR Manservigi
Nov 18, 2008·The Journal of Biological Chemistry·Dustin VerpootenBin He
Feb 22, 2012·Journal of Virology·Ryuichi KanaiSamuel D Rabkin
Nov 3, 2006·Journal of Virology·Stephanie A CampbellMatthias Gromeier
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·S TanejaI Mohr
Sep 25, 2010·Seminars in Immunopathology·Hee Jin KimJae U Jung
Jan 26, 2007·Expert Opinion on Biological Therapy·Giulia Fulci, E Antonio Chiocca
Dec 24, 2003·International Reviews of Immunology·Ian Mohr
Oct 16, 2015·Molecular Therapy Oncolytics·Cole Peters, Samuel D Rabkin
Dec 3, 2002·Nature Reviews. Cancer·E Antonio Chiocca
Mar 3, 2017·Journal of Virology·Valerio LeoniGabriella Campadelli-Fiume
Sep 15, 2017·Journal of Virology·Richard ManivanhDavid A Leib
Sep 15, 2009·Reviews in Medical Virology·Sagar B Kudchodkar, Beth Levine
Apr 13, 2007·Gene Therapy·A C ShahK A Cassady
Mar 5, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Jia-Ni WangRen-Rin Liu
Jan 1, 2016·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Frederick J Kohlhapp, Howard L Kaufman
Nov 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jennifer C HuTahereh Kamalati
Nov 11, 2020·International Journal of Molecular Sciences·Laura Menotti, Elisa Avitabile
Apr 1, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Megan J DambachNancy S Markovitz
Nov 23, 2013·Journal of Molecular Biology·Yijie Ma, Bin He

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