Apr 28, 2020

Efficient Dilution-to-Extinction isolation of novel virus-host model systems for fastidious heterotrophic bacteria

BioRxiv : the Preprint Server for Biology
H. H. BuchholzBen Temperton


Microbes and their associated viruses are key drivers of biogeochemical processes in marine and soil biomes. While viruses of phototrophic cyanobacteria are well-represented in model systems, challenges of isolating marine microbial heterotrophs and their viruses have hampered attempts to study the Earth's most abundant virus-host systems. A recent resurgence in cultivation efforts has improved the cultivability of fastidious bacteria for hypothesis testing and synthetic community ecology. Yet, these improvements have not been matched by similar efforts to cultivate their associated bacteriophages. Here, we describe a high-throughput method for isolating ecologically important virus-host systems for fastidious heterotrophic bacteria, that couples advances in Dilution-to-Extinction culturing of hosts with sequential enrichment and isolation of associated phages, bringing viral cultivation efforts in line with those of their cellular hosts. Applied to five monthly samples from the Western English Channel, we successfully isolated 117 new phages including the first known siphovirus infecting SAR11 and the first known phages infecting members of the methylotrophic bacterial clade OM43. Genomic analyses of 13 novel viruses revealed ...Continue Reading

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Mentioned in this Paper

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