A High-Affinity Fluorescent Sensor for Catecholamine: Application to Monitoring Norepinephrine Exocytosis

Angewandte Chemie
Le ZhangTimothy E Glass

Abstract

A fluorescent sensor for catecholamines, NS510, is presented. The sensor is based on a quinolone fluorophore incorporating a boronic acid recognition element that gives it high affinity for catecholamines and a turn-on response to norepinephrine. The sensor results in punctate staining of norepinephrine-enriched chromaffin cells visualized using confocal microscopy indicating that it stains the norepinephrine in secretory vesicles. Amperometry in conjunction with total internal reflection fluorescence (TIRF) microscopy demonstrates that the sensor can be used to observe destaining of individual chromaffin granules upon exocytosis. NS510 is the highest affinity fluorescent norepinephrine sensor currently available and can be used for measuring catecholamines in live-cell assays.

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Citations

Oct 16, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ying WenCaixia Yin
Mar 11, 2020·Annual Review of Analytical Chemistry·Jacqueline D KeighronAnn-Sofie Cans
Aug 12, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lihe ZhaoDaqian Song
Jan 17, 2020·ChemPlusChem·Justine PandardManon Guille-Collignon
Dec 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Zhong-Hui WangQi Lin
Feb 1, 2020·The Journal of Organic Chemistry·Yota SuzukiKoji Ishihara
Nov 14, 2019·Analytical Chemistry·Qichao YaoXiaojun Peng
Oct 6, 2021·Nature Communications·Wendong JiaShuo Huang

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