A high-affinity interaction with ADP-actin monomers underlies the mechanism and in vivo function of Srv2/cyclase-associated protein

Molecular Biology of the Cell
Pieta K MattilaBruce L Goode

Abstract

Cyclase-associated protein (CAP), also called Srv2 in Saccharomyces cerevisiae, is a conserved actin monomer-binding protein that promotes cofilin-dependent actin turnover in vitro and in vivo. However, little is known about the mechanism underlying this function. Here, we show that S. cerevisiae CAP binds with strong preference to ADP-G-actin (Kd 0.02 microM) compared with ATP-G-actin (Kd 1.9 microM) and competes directly with cofilin for binding ADP-G-actin. Further, CAP blocks actin monomer addition specifically to barbed ends of filaments, in contrast to profilin, which blocks monomer addition to pointed ends of filaments. The actin-binding domain of CAP is more extensive than previously suggested and includes a recently solved beta-sheet structure in the C-terminus of CAP and adjacent sequences. Using site-directed mutagenesis, we define evolutionarily conserved residues that mediate binding to ADP-G-actin and demonstrate that these activities are required for CAP function in vivo in directing actin organization and polarized cell growth. Together, our data suggest that in vivo CAP competes with cofilin for binding ADP-actin monomers, allows rapid nucleotide exchange to occur on actin, and then because of its 100-fold weak...Continue Reading

References

Mar 1, 1991·Molecular and Cellular Biology·J E GerstJ Field
Nov 17, 1986·European Journal of Biochemistry·A G WeedsJ Gooch
Jan 1, 1982·Methods in Cell Biology·J D Pardee, J A Spudich
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M F CarlierD Pantaloni
May 1, 1996·Analytical Biochemistry·J PeränenL Kääriäinen
Jun 1, 1996·Journal of Cellular Biochemistry·A HubbersteyD Young
Sep 17, 1998·The Journal of Biological Chemistry·L Blanchoin, T D Pollard
Jun 8, 1999·The Journal of Biological Chemistry·G VaduvaN Ramesh
Jun 15, 1999·The Journal of Cell Biology·A A RodalD C Amberg
Aug 23, 2000·The Journal of Cell Biology·A K WolvenD G Drubin
Nov 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·H YamaguchiT Takenawa
Jan 9, 2001·Nature Cell Biology·J B MarchandH N Higgs
Aug 8, 2001·The Journal of Cell Biology·B L DreesD G Drubin
Feb 22, 2002·The Journal of Biological Chemistry·Maud HertzogMarie-France Carlier
Mar 26, 2002·FEBS Letters·Eija PaunolaPekka Lappalainen
Mar 29, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Andrew V Hubberstey, Emilio P Mottillo
Nov 14, 2002·Molecular Biology of the Cell·Pauli J OjalaPekka Lappalainen
Nov 5, 2003·Molecular Biology of the Cell·Angelika A NoegelCornelis J Weijer
Mar 9, 2004·Molecular Biology of the Cell·Enni BertlingPekka Lappalainen
Aug 18, 2004·Biochemistry·Tetyana DodatkoSteven C Almo

❮ Previous
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Citations

Nov 26, 2013·European Journal of Cell Biology·Bo Xue, Robert C Robinson
Dec 19, 2009·Cell Death and Differentiation·J E LeadshamC W Gourlay
Jun 1, 2007·Molecular Biology of the Cell·Faisal ChaudhryChristopher J Staiger
Aug 18, 2006·Molecular and Cellular Biology·C W Gourlay, K R Ayscough
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·James B Moseley, Bruce L Goode
May 4, 2006·Annual Review of Plant Biology·Patrick J HusseyMichael J Deeks
Mar 25, 2010·Journal of Cell Science·Meng-Chi LinJohn A Cooper
Mar 21, 2007·The Journal of Cell Biology·Pieta K MattilaPekka Lappalainen
Jan 25, 2011·European Journal of Cell Biology·Julia Magdalena SattlerHerwig Schüler
Sep 9, 2008·Trends in Cell Biology·Louis RenaultMarie-France Carlier
Oct 13, 2015·Nature Cell Biology·Adam B JohnstonBruce L Goode
Sep 17, 2008·Molecular Microbiology·T V Satish TammanaChhitar M Gupta
Jul 7, 2015·Cytoskeleton·Casey A YdenbergBruce L Goode
Oct 3, 2006·Current Opinion in Plant Biology·Christopher J Staiger, Laurent Blanchoin
Sep 5, 2006·Journal of Molecular Biology·Adlina Mohd YusofAndreas Hofmann
May 12, 2005·Current Biology : CB·Susan Nicholson-DykstraElizabeth S Harris
Aug 6, 2011·Molecular Cell·Clotilde HussonMarie-France Carlier
Aug 11, 2016·Frontiers in Cellular Neuroscience·Atul KumarVivek S Peche
Mar 15, 2018·Biochemistry. Biokhimii︠a︡·G V KakurinaI V Kondakova
Sep 18, 2014·The Journal of Biological Chemistry·Silvia JansenBruce L Goode
Jul 11, 2008·The Biochemical Journal·Vernonica E Franklin-Tong, Campbell W Gourlay
Nov 14, 2019·International Journal of Molecular Sciences·Vedud PurdeDmitri S Kudryashov
May 16, 2018·Nature Communications·Tommi KotilaPekka Lappalainen

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