A high density of ancient spliceosomal introns in oxymonad excavates.

BMC Evolutionary Biology
Claudio H Slamovits, Patrick J Keeling

Abstract

Certain eukaryotic genomes, such as those of the amitochondriate parasites Giardia and Trichomonas, have very low intron densities, so low that canonical spliceosomal introns have only recently been discovered through genome sequencing. These organisms were formerly thought to be ancient eukaryotes that diverged before introns originated, or at least became common. Now however, they are thought to be members of a supergroup known as excavates, whose members generally appear to have low densities of canonical introns. Here we have used environmental expressed sequence tag (EST) sequencing to identify 17 genes from the uncultivable oxymonad Streblomastix strix, to survey intron densities in this most poorly studied excavate group. We find that Streblomastix genes contain an unexpectedly high intron density of about 1.1 introns per gene. Moreover, over 50% of these are at positions shared between a broad spectrum of eukaryotes, suggesting they are very ancient introns, potentially present in the last common ancestor of eukaryotes. The Streblomastix data show that the genome of the ancestor of excavates likely contained many introns and the subsequent evolution of introns has proceeded very differently in different excavate lineage...Continue Reading

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Feb 12, 2008·Nucleic Acids Research·Manuel Irimia, Scott William Roy
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Datasets Mentioned

BETA
AAM67532
DQ363679

Methods Mentioned

BETA
PCR

Software Mentioned

BLASTX
Streblomastix
PEPdb

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