A high-fat diet impairs mitochondrial biogenesis, mitochondrial dynamics, and the respiratory chain complex in rat myocardial tissues

Journal of Cellular Biochemistry
Dan ChenLing Gao

Abstract

A high-fat diet (HFD) has been associated with heart failure and arrhythmias; however, the molecular mechanisms underlying these associations are poorly understood. The mitochondria play an essential role in optimal heart performance, most of the energy for which is obtained from the oxidation of fatty acids. As such, chronic exposure to excess fatty acids may cause mitochondrial dysfunction and heart failure. To investigate the effects of a HFD on the mitochondrial function in the myocardium, 40 male rats were randomly divided into two groups and fed with either a normal diet or a HFD for 28 weeks. The myocardial lipid content, cardiac parameters and function, and mitochondrial morphology and function were evaluated. The expression of a number of genes involved in mitochondrial dynamics was measured using quantitative polymerase chain reaction and Western blot analyses. Proteomic analysis was also performed to identify the proteins affected by HFD treatment. Significant fat deposition in the myocardia, cardiac hypertrophy, and cardiac dysfunction were all observed in HFD-treated rats. Electron microscopy showed abnormal mitochondrial density and morphology. In addition, abnormal expression of genes involved in mitochondrial dy...Continue Reading

References

Apr 20, 2004·Journal of the American College of Cardiology·Linda R PetersonVíctor G Dávila-Román
Mar 18, 2008·Cardiovascular Research·David J Chess, William C Stanley
Jul 9, 2009·Physiological Reviews·Marc LiesaAntonio Zorzano
Sep 29, 2009·Biochimica Et Biophysica Acta·Patrick SchrauwenMatthijs K C Hesselink
Jan 21, 2010·Physiological Reviews·Gary D LopaschukWilliam C Stanley
Dec 15, 2010·Cell·Markus R Wenk
Jan 20, 2011·Molecular and Cellular Biology·Jessica M EllisRosalind A Coleman
Jun 2, 2011·Chemical Reviews·Rosalind A Coleman, Douglas G Mashek
Mar 3, 2012·Circulation Research·William C StanleyKelly A O'Connell
Jul 17, 2012·Antioxidants & Redox Signaling·Sang-Bing OngDerek J Hausenloy
Oct 17, 2012·International Journal of Obesity : Journal of the International Association for the Study of Obesity·O J RiderS Neubauer
Apr 30, 2014·Acta Physiologica·M van Bilsen, A Planavila
May 9, 2014·Biochimica Et Biophysica Acta·Sidney Y Ren, Xihui Xu
Oct 12, 2014·Journal of Molecular and Cellular Cardiology·Yoshiyuki IkedaJunichi Sadoshima
Mar 13, 2015·Antioxidants & Redox Signaling·Joel D Schilling
Apr 12, 2015·EMBO Molecular Medicine·Gerald W Dorn
Nov 6, 2015·The Journal of Physiology·César Vasquez-TrincadoSergio Lavandero
Dec 23, 2016·Nature Reviews. Cardiology·David A BrownMihai Gheorghiade
Jan 22, 2017·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Nan Hu, Yingmei Zhang
Jul 21, 2017·JCI Insight·Ruiping JiP Christian Schulze

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Citations

Sep 1, 2019·Current Pharmaceutical Design·Caroline D VelosoPaulo J Oliveira
Dec 7, 2019·Clinical Science·Chayodom ManeechoteNipon Chattipakorn
Jan 29, 2020·European Journal of Clinical Investigation·Ching-Yi ChenShih-Torng Ding
Dec 19, 2019·International Journal of Molecular Sciences·Matthew C ValdezPrasada Rao S Kodavanti
Oct 23, 2020·Antioxidants·Blake H GowenJohn P Morrow
Feb 25, 2021·Insect Biochemistry and Molecular Biology·Robert J CormierNicolas Pichaud
Jun 9, 2021·GeroScience·Ana P ValenciaDavid J Marcinek
Sep 4, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Satya Murthy TadinadaE Dale Abel

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Methods Mentioned

BETA
protein assay
electrophoresis
PCR

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