PMID: 11934434Apr 6, 2002Paper

A high frequency of mtDNA polymorphisms in HeLa cell sublines

Mutation Research
Corinna HerrnstadtN Howell

Abstract

The complete mtDNA sequences from the uncloned "founder" HeLa cells and from five sublines have been determined. These sequences all carry a common "core" of 38 single basepair alterations relative to the revised Cambridge Reference Sequence (CRS). The HeLa mitochondrial genome is of African descent and it is a member of the African L3 haplogroup. The sequence of the HeLa mtDNA resolves the uncertainty surrounding the mosaic composition of the original CRS for human mtDNA. Most importantly, we detected a total of eight polymorphisms that have arisen in the mtDNA coding region of different HeLa sublines. These observations suggest that HeLa mtDNA has a high rate of sequence divergence, relative to the phylogenetically-derived divergence rate for mtDNAs in the human population, which results from a relaxation of negative selection against the fixation of deleterious mutations. Furthermore, this high frequency of polymorphisms in HeLa mtDNA may reflect a process similar to the accumulation of somatic mtDNA mutations in human cancers. Preliminary analysis of single-cell derived subclone lines revealed the occurrence of another polymorphism and provided evidence for a large number of mtDNA segregation units.

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Citations

Mar 6, 2003·Mechanisms of Ageing and Development·Yevgenya KraytsbergKonstantin Khrapko
Feb 1, 2003·Nucleic Acids Research·Penelope A MasonRobert N Lightowlers
Sep 5, 2003·Nucleic Acids Research·María Pilar Bayona-BafaluyJosé Antonio Enríquez
Dec 5, 2002·Journal of Neuro-ophthalmology : the Official Journal of the North American Neuro-Ophthalmology Society·Neil HowellIwona Kubacka
Feb 8, 2007·Genome Research·Ana GoiosAntónio Amorim
Oct 7, 2003·Annual Review of Genomics and Human Genetics·Sarah A Tishkoff, Brian C Verrelli
Aug 25, 2010·Biochimica Et Biophysica Acta·María Pilar Bayona-BafaluyEduardo Ruiz-Pesini
Aug 27, 2016·Mitochondrion·Christine Sabine SiegismundDirk Lassner
Nov 5, 2003·The Journal of Clinical Investigation·Robert W TaylorDouglass M Turnbull
Jun 15, 2017·Journal of the American Chemical Society·Takuya HidakaHiroshi Sugiyama
Oct 2, 2003·Annals of Neurology·Robert W TaylorDouglass M Turnbull
Jun 21, 2005·Movement Disorders : Official Journal of the Movement Disorder Society·Maher A NoureddineMichael A Hauser
Jul 5, 2019·International Journal of Molecular Sciences·Matthew J YoungCarolyn K J Young

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