A high-molecular-weight fraction of smooth lipopolysaccharide in Klebsiella serotype O1:K20 contains a unique O-antigen epitope and determines resistance to nonspecific serum killing.

Infection and Immunity
K L McCallumC Whitfield

Abstract

The lipopolysaccharide (LPS) O-antigen side chains of Klebsiella serotype O1 have been studied by using mutants selected by resistance to a Klebsiella bacteriophage designated O1-A. Two classes of LPS mutants were identified. The major group (90%) synthesized rough LPS. The remaining 10% of the mutants produced a novel LPS profile that lacked the highest-molecular-weight O-substituted molecules (HMW-LPS) but still produced lower-molecular-weight O-substituted species (LMW-LPS). By using antisera raised against mutant Klebsiella strains and antiserum specific for Pasteurella haemolytica serotype 4, it was demonstrated that HMW-LPS and LMW-LPS contain shared epitopes. HMW-LPS also contained an epitope absent in LMW-LPS. This unique epitope was recognized by a monoclonal antibody (O1-52.6) and appears to be responsible for the serological cross-reaction between the O antigens of Klebsiella O1 and Escherichia coli O19. This HMW-LPS epitope was present in eight other Klebsiella O1 isolates which were examined. Electron microscopy demonstrated that HMW-LPS excluded overlying capsular polysaccharide for a distance of 25 to 40 nm. The distance was reduced to 10 to 18 nm in strains which synthesized only LMW-LPS and to zero in rough LPS...Continue Reading

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Citations

May 20, 2020·The Journal of Biological Chemistry·Chris WhitfieldSteven D Kelly
Jan 19, 2016·Frontiers in Cellular and Infection Microbiology·Marcos Mancilla
Nov 30, 2019·Frontiers in Cellular and Infection Microbiology·L Ponoop Prasad Patro, Thenmalarchelvi Rathinavelan
Mar 25, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Dung Q NguyenHiroshi Shiigi
Apr 1, 2018·The Journal of Biological Chemistry·Bradley R ClarkeChris Whitfield
Sep 1, 1995·Clinical and Diagnostic Laboratory Immunology·H L CurrieJ S Lam
Sep 25, 1997·Clinical and Diagnostic Laboratory Immunology·M TrautmannC Whitfield

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