A high-performance liquid chromatography method for determination of 4-aminopyridine in tissues and urine

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
S W Casteel, B R Thomas

Abstract

An analytical method was developed to measure 4-aminopyridine in tissues and urine to determine appropriate diagnostic samples in acute poisoning cases. Tissues from rats dosed with 4-aminopyridine were extracted with methylene chloride. Extracts were analyzed by high-performance liquid chromatography using an isocratic solvent system of acetonitrile and aqueous solution (15/85 v/v) consisting of 0.015 M sodium salt of l-heptane-sulfonic acid, 0.002 M tetramethylammonium bromide, and 0.01 M sodium dihydrogen phosphate adjusted to pH 3.0 with phosphoric acid. We concluded that suitable diagnostic samples for acute poisoning cases include stomach contents, kidney, liver, and urine.

References

Jun 1, 1975·Bulletin of Environmental Contamination and Toxicology·J E Peterson
Jun 1, 1980·Clinical Toxicology·D A SpykerJ A Sinn
Jul 9, 1982·Journal of Chromatography·Y ShinoharaN Castagnoli

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