A high performance system to study the influence of temperature in on-line solid-phase extraction capillary electrophoresis

Analytica Chimica Acta
Marcos TasconLeonardo G Gagliardi

Abstract

A novel high performance system to control the temperature of the microcartridge in on-line solid phase extraction capillary electrophoresis (SPE-CE) is introduced. The mini-device consists in a thermostatic bath that fits inside of the cassette of any commercial CE instrument, while its temperature is controlled from an external circuit of liquid connecting three different water baths. The circuits are controlled from a switchboard connected to an array of electrovalves that allow to rapidly alternate the water circulation through the mini-thermostatic-bath between temperatures from 5 to 90 °C. The combination of the mini-device and the forced-air thermostatization system of the commercial CE instrument allows to optimize independently the temperature of the sample loading, the clean-up, the analyte elution and the electrophoretic separation steps. The system is used to study the effect of temperature on the C18-SPE-CE analysis of the opioid peptides, Dynorphin A (Dyn A), Endomorphin1 (END) and Met-enkephalin (MET), in both standard solutions and in spiked plasma samples. Extraction recoveries demonstrated to depend, with a non-monotonous trend, on the microcartridge temperature during the sample loading and became maximum at ...Continue Reading

References

Mar 4, 2005·Analytical Chemistry·Norberto A Guzman, Terry M Phillips
Aug 16, 2006·Analytical Chemistry·Leonardo G GagliardiElisabeth Bosch
Oct 27, 2007·Electrophoresis·Elena HernándezJosé Barbosa
Mar 4, 2009·Analytical and Bioanalytical Chemistry·Agnes T AranasJoselito P Quirino
Feb 27, 2010·Journal of Separation Science·Fernando BenaventeVictoria Sanz-Nebot
Dec 21, 2010·Electrophoresis·Zdena MaláPetr Boček
Oct 12, 2013·Electrophoresis·Rawi RamautarGerhardus J de Jong

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Citations

Oct 16, 2015·Electrophoresis·Rawi RamautarGerhardus J de Jong
Sep 8, 2015·Analytica Chimica Acta·Leonardo G GagliardiCecilia B Castells

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