A high-performance thin-layer chromatographic technique to screen cocaine in urine samples

Legal Medicine
Mauricio Yonamine, Mônica Cortez Sampaio

Abstract

Qualitative identification of cocaine and its metabolites in urine samples is generally carried out by an immunoassay technique followed by a gas chromatographic/mass spectrometric confirmation of presumptive positives. Nevertheless, other chromatographic techniques such as thin-layer chromatography or gas chromatography could also be used to screen several types of drugs of abuse especially for forensic and legal purposes. In the present work, the capability of high performance thin-layer chromatography (HPTLC) to detect cocaine in urine samples and discriminate it from interfering substances (local anaesthetic, caffeine and nicotine) was studied. Twenty urine samples of drug addicts were submitted to the HPTLC method. Unaltered cocaine present in the urine samples and cocaine obtained after methylation of benzoylecgonine (main cocaine metabolite) with diazomethane were detected in all tested samples. In conclusion, the proposed HPTLC method showed to be useful to detect cocaine in biological matrices.

References

Nov 26, 1975·Journal of Chromatography·J E WallaceK Blum
Mar 1, 1980·Clinical Toxicology·R D BuddF C Yang
May 17, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maurício Yonamine, Ovandir Alves Silva
Sep 20, 2005·Journal of Analytical Toxicology·Emiko KurisakiKouichi Hiraiwa

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Citations

Dec 16, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Mei HuaYunhui Yang

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