PMID: 8587804Jan 1, 1996Paper

A high prevalence of mixed trypanosome infections in tsetse flies in Sinfra, Côte d'Ivoire, detected by DNA amplification

Parasitology
D K MasigaW Gibson

Abstract

The prevalence of various species and subgroups of trypanosomes in the Sinfra area of Côte d'Ivoire was determined using the polymerase chain reaction (PCR). Using this technique to amplify specific satellite DNA targets, it was possible to identify developmental-stage trypanosomes in the midguts and the proboscides of tsetse without expansion of parasite populations. The predominant tsetse species in the area was Glossina palpalis, while G. pallicera and G. nigrofusca were also present. Microscopical examination of 811 non-teneral flies revealed an infection rate of 14% in midguts and/or proboscides. Three subgroups of Trypanosoma congolense (Savannah, Forest & Kilifi), T. simiae, T. godfreyi, West African T. vivax and T. brucei ssp. were identified using PCR. T. congolense Forest was the most abundant of the Nannomonas trypanosomes. Approximately 40% of all infections were mixed, and there was a significantly higher prevalence of apparently mature T. brucei ssp. trypanosomes than has previously been reported. The present study demonstrates that PCR facilitates the easy identification of mature trypanosome infections in tsetse, providing a reliable estimation of trypanosomiasis challenge.

References

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Citations

Mar 17, 2004·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Z K NjiruW C Gibson
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Wendy Gibson
Dec 10, 1999·Molecular and Biochemical Parasitology·N Downey, J E Donelson
Sep 11, 1999·Parasitology Today·S C Welburn, I Maudlin
Dec 31, 1998·Veterinary Parasitology·P J Pereira de AlmeidaS Osaer
Nov 26, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Imna MaleleWendy Gibson
Sep 8, 2007·American Journal of Respiratory and Critical Care Medicine·Ellen A van der EijkJaap T van Dissel
Nov 14, 2003·Kinetoplastid Biology and Disease·Wendy Gibson
Jun 15, 2010·Trends in Parasitology·John C EnyaruDaniel K Masiga
Nov 17, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Gustave SimoJörg Hoheisel
Mar 22, 2007·Veterinary Parasitology·J L GonzalesL M Siles
Feb 1, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Adriana C RodriguesMarta M G Teixeira
Nov 10, 2001·Annual Review of Genomics and Human Genetics·A V Hill
Aug 18, 2018·Parasite : Journal De La Société Française De Parasitologie·Sartrien Kanté TagueuGustave Simo
Feb 20, 2016·PLoS Neglected Tropical Diseases·Lucas J CunninghamEmily R Adams
Nov 7, 2001·The Journal of Parasitology·P SolanoF Thomas
Mar 31, 2009·International Journal of Systematic and Evolutionary Microbiology·Vyacheslav Y YurchenkoDmitri A Maslov
Oct 1, 1996·Annals of Tropical Medicine and Parasitology·J P Gouteux, W C Gibson
Jun 3, 2015·Parasite : Journal De La Société Française De Parasitologie·Vincent DjohanPhilippe Solano
Jul 10, 2017·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Herakles A GarciaMarta M G Teixeira

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