A high quantum yield molecule-protein complex fluorophore for near-infrared II imaging

Nature Communications
Alexander L AntarisZhen Cheng

Abstract

Fluorescence imaging in the second near-infrared window (NIR-II) allows visualization of deep anatomical features with an unprecedented degree of clarity. NIR-II fluorophores draw from a broad spectrum of materials spanning semiconducting nanomaterials to organic molecular dyes, yet unfortunately all water-soluble organic molecules with >1,000 nm emission suffer from low quantum yields that have limited temporal resolution and penetration depth. Here, we report tailoring the supramolecular assemblies of protein complexes with a sulfonated NIR-II organic dye (CH-4T) to produce a brilliant 110-fold increase in fluorescence, resulting in the highest quantum yield molecular fluorophore thus far. The bright molecular complex allowed for the fastest video-rate imaging in the second NIR window with ∼50-fold reduced exposure times at a fast 50 frames-per-second (FPS) capable of resolving mouse cardiac cycles. In addition, we demonstrate that the NIR-II molecular complexes are superior to clinically approved ICG for lymph node imaging deep within the mouse body.

References

Jul 1, 1976·Biophysical Chemistry·J B Ifft
Jan 1, 1978·Physics in Medicine and Biology·R C Benson, H A Kues
May 2, 1998·Microvascular Research·S MordonT Desmettre
Mar 9, 2006·Journal of the American Chemical Society·James E MurphyArthur J Nozik
Jan 16, 2008·Journal of the American Chemical Society·Keitaro UmezawaKoji Suzuki
Feb 21, 2009·Molecular Pharmaceutics·Anne-Katrin KirchherrKarsten Mäder
Sep 4, 2009·Acta Ophthalmologica·Tetsuya SugiyamaSelim Orgul
Nov 7, 2009·Nature Nanotechnology·Kevin WelsherHongjie Dai
Mar 10, 2010·Journal of Biomedical Optics·Song Hu, Lihong V Wang
Jun 10, 2011·Angewandte Chemie·Hak Soo ChoiJohn V Frangioni
Oct 4, 2011·American Journal of Obstetrics and Gynecology·Merlijn HuttemanAlexander L Vahrmeijer
Oct 5, 2012·Journal of the American Chemical Society·Shuo DiaoHongjie Dai
Nov 20, 2012·Nature Medicine·Guosong HongHongjie Dai
Jan 8, 2013·Nature Biotechnology·Hak Soo ChoiJohn V Frangioni
Feb 2, 2013·IEEE Reviews in Biomedical Engineering·Janaka SenarathnaNitish V Thakor
Nov 5, 2013·Nature Nanotechnology·Nicole M IversonMichael S Strano
Mar 25, 2014·Circulation. Cardiovascular Imaging·Guosong HongNgan F Huang
May 9, 2014·Journal of Biomedical Optics·Laura A SordilloRobert R Alfano
Sep 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Debadyuti GhoshAngela M Belcher
Sep 18, 2014·Nature Communications·Neelkanth M BardhanAngela M Belcher
Oct 16, 2015·Angewandte Chemie·Shuo DiaoHongjie Dai
Nov 26, 2015·Nature Materials·Alexander L AntarisHongjie Dai
Nov 26, 2015·Chemical Communications : Chem Comm·Shahi Imam RejaManoj Kumar
Apr 27, 2016·Proceedings of the National Academy of Sciences of the United States of America·Xiangnan DangPaula T Hammond
May 24, 2016·Chemistry of Materials : a Publication of the American Chemical Society·Yifei KongDejian Zhou
Sep 1, 2014·Nature Photonics·Guosong HongHongjie Dai

❮ Previous
Next ❯

Citations

Feb 23, 2018·Advanced Materials·Kevin J McHughAna Jaklenec
Jul 4, 2018·Advanced Healthcare Materials·Ji QiBen Zhong Tang
Jul 28, 2018·Advanced Healthcare Materials·Xiaodong ZengXuechuan Hong
Aug 15, 2017·Angewandte Chemie·Emily D CoscoEllen M Sletten
Sep 6, 2018·Advanced Materials· KenryBin Liu
Sep 25, 2018·Chembiochem : a European Journal of Chemical Biology·Yawei MiaoHailin Cong
Feb 12, 2019·Chemical Communications : Chem Comm·Xiaodong ZengYuling Xiao
Apr 27, 2019·Advanced Materials·Shoujun ZhuHongjie Dai
Dec 4, 2019·Advanced Healthcare Materials·Hui ZhouXuechuan Hong
Mar 4, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xue ZhangShiping Yang
Mar 20, 2020·Molecular Oncology·Xiao ZhangXiaoli Lan
May 15, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Thines KanagasundaramKlaus Kopka
Jun 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Mingxi ZhangHongjie Dai
Sep 28, 2018·Advanced Healthcare Materials·Xiaowen LiuZhuang Liu
Sep 29, 2019·Journal of Cellular and Molecular Medicine·Leyla Fath-BayatiEhsan Sharif-Paghaleh
Dec 11, 2019·Chemical Communications : Chem Comm·Kun QianZhen Cheng
Jan 17, 2020·Advanced Healthcare Materials·Ling'e ZhangXiqun Jiang
Dec 31, 2019·Advanced Functional Materials·Hao WanHongjie Dai

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence imaging
nuclear magnetic resonance
fluorescence titration
imaging technique
light scattering
with

Software Mentioned

Image J
LabView

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.