A high-resolution HPLC-QqTOF platform using parallel reaction monitoring for in-depth lipid discovery and rapid profiling

Analytica Chimica Acta
Dingyi YuUte Roessner

Abstract

Here, we developed a robust lipidomics workflow merging both targeted and untargeted approaches on a single liquid chromatography coupled to quadrupole-time of flight (LC-QqTOF) mass spectrometry platform with parallel reaction monitoring (PRM). PRM assays integrate both untargeted profiling from MS1 scans and targeted profiling obtained from MS/MS data. This workflow enabled the discovery of more than 2300 unidentified features and identification of more than 600 lipid species from 23 lipid classes at the level of fatty acid/long chain base/sterol composition in a barley root extracts. We detected the presence of 142 glycosyl inositol phosphorylceramides (GIPC) with HN(Ac)-HA as the core structure of the polar head, 12 cardiolipins and 17 glucuronosyl diacylglycerols (GlcADG) which have been rarely reported previously for cereal crops. Using a scheduled algorithm with up to 100 precursors multiplexed per duty cycle, the PRM assay was able to achieve a rapid profiling of 291 species based on MS/MS data by a single injection. We used this novel approach to demonstrate the applicability and efficiency of the workflow to study salt stress induced changes in the barley root lipidome. Results show that 221 targeted lipids and 888 un...Continue Reading

Citations

Aug 24, 2019·Metabolomics : Official Journal of the Metabolomic Society·Saeedur RahmanSyed Ghulam Musharraf
Apr 20, 2020·Metabolomics : Official Journal of the Metabolomic Society·Jeanaflor Crystal T ConcepcionMelissa A Fitzgerald
Apr 17, 2020·Electrophoresis·C D CalvanoT R I Cataldi
Mar 3, 2020·Frontiers in Plant Science·Dingyi YuThusitha W T Rupasinghe
Dec 29, 2019·Scientific Reports·Tejia ZhangRandall T Peterson
Dec 18, 2020·Frontiers in Plant Science·Da CaoChristine A Beveridge
Nov 23, 2018·Progress in Lipid Research·Adiilah Mamode CassimSébastien Mongrand
Apr 19, 2021·The Plant Journal : for Cell and Molecular Biology·Cheka KehelpannalaUte Roessner
Mar 5, 2020·Journal of the American Society for Mass Spectrometry·Jone GarateJosé A Fernández
Oct 27, 2021·Molecular Omics·Cheka KehelpannalaUte Roessner

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