A high-resolution large area serotonin map of a live rat brain section

Neuroreport
Sanjeev K KaushalyaSudipta Maiti

Abstract

We employ three-photon microscopy to produce a high-resolution map of serotonin autofluorescence in a rat midbrain section (covering more than half of the brain), to quantitatively characterize serotonin distribution and release in different areas of a live brain slice. The map consists of a tiling of approximately 160 contiguous optical images (covering an area of approximately 27 mm with sub-mum resolution in 20 min), and is recorded before and after inducing depolarization. We observe that the total serotonin exocytosed from the somata in the raphe is quantitatively comparable with regions containing a high density of serotonergic processes. Our results demonstrate that high-resolution, wide-area, dynamic neurotransmitter mapping is now possible.

References

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Citations

Dec 18, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lesley A ColganEdwin S Levitan
Mar 26, 2014·ACS Chemical Neuroscience·Bidyut SarkarSudipta Maiti
May 27, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Anand Kant DasSudipta Maiti
Jan 9, 2019·Frontiers in Neuroscience·Emily QuentinLuc Maroteaux
Aug 11, 2017·ACS Chemical Neuroscience·Anand Kant DasSudipta Maiti

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