Abstract
The development of a highly reliable physical map with landmark sites spaced an average of 100 kbp apart has been a central goal of the Human Genome Project. We have approached the physical mapping of human chromosome 11 with this goal as a primary target. We have focused on strategies that would utilize yeast artificial chromosome (YAC) technology, thus permitting long-range coverage of hundreds of kilobases of genomic DNA, yet we sought to minimize the ambiguities inherent in the use of this technology, particularly the occurrence of chimeric genomic DNA clones. This was achieved through the development of a chromosome 11-specific YAC library from a human somatic cell hybrid line that has retained chromosome 11 as its sole human component. To maximize the efficiency of YAC contig assembly and extension, we have employed an Alu-PCR-based hybridization screening system. This system eliminates many of the more costly and time-consuming steps associated with sequence tagged site content mapping such as sequencing, primer production, and hierarchical screening, resulting in greater efficiency with increased throughput and reduced cost. Using these approaches, we have achieved YAC coverage for >90% of human chromosome 11, with an a...Continue Reading
References
Oct 2, 1992·Science·S FooteD C Page
Oct 1, 1992·Nature·I ChumakovL Grinas
Oct 1, 1992·Genomics·J KereD Schlessinger
Dec 1, 1991·Genomics·R ArratiaM S Waterman
Nov 1, 1991·PCR Methods and Applications·E D Green, P Green
Apr 25, 1990·Nucleic Acids Research·R AnandA F Markham
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E D Green, M V Olson
Mar 20, 1995·Genomics·J LiuE Schurr
Jan 1, 1995·Nature Genetics·T G ThompsonJ J Wasmuth
Jul 15, 1994·Genomics·J KunzK H Grzeschik
Sep 1, 1994·Genomics·M J HigginsB W Kim
Feb 1, 1994·Genomics·D J MunroeD E Housman
Sep 1, 1993·Genomics·M W SmithY Lin
Citations
May 26, 1999·The Journal of Pathology·J KorethJ O McGee
Jan 22, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M LenerT Meo
Mar 20, 1999·Mutation Research·C A WaldrenJ F Sinclair
Dec 5, 1998·Biochimica Et Biophysica Acta·D S O'KeefeW D Heston
Mar 21, 1998·Nature Genetics·V G CheungR S Spielman
May 30, 2001·European Journal of Human Genetics : EJHG·K LuS B Patel
Dec 30, 1999·Genome Research·E T PetersonD L Grady
Sep 23, 1997·Genomics·B E BaysalC W Richard
Jul 1, 2004·Mutation Research·Charles A WaldrenAkiko M Ueno
Oct 21, 2003·Radiation Research·M LenarczykC A Waldren
Apr 15, 1997·Genomics·C AndrewsK Roeder
Apr 15, 1997·Genomics·P R CooperT B Shows
Jun 15, 1997·Genomics·S C GuruS C Chandrasekharappa
Aug 1, 1997·Genomics·L H ReidM J Higgins
Nov 5, 1997·Genomics·B WatsonD G Warnock
Jul 9, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Carley D RossMichael H Fox
Apr 21, 2001·European Journal of Human Genetics : EJHG·B E BaysalB Devlin
May 29, 2000·Radiation Research·B FouladiP K Cooper
Jun 22, 2001·Radiation Research·S M KraemerC A Waldren
Apr 9, 2008·Physiological Reviews·H Criss HartzellLi-Ting Chien
Feb 18, 2004·Molecular and Cellular Biology·Nicolas PineaultR Keith Humphries
Mar 1, 1997·Neuropsychology Review·D N AllenB E Seaton
Aug 1, 1997·Genome Research·C M SmithD S Gerhard
Jul 1, 1997·Genome Research·G G BouffardE D Green
Feb 21, 1998·Genome Research·H StöhrB H Weber
Mar 21, 1998·Genome Research·S L ChristianD H Ledbetter
Feb 21, 1998·Genome Research·M J HigginsT B Shows
Mar 21, 1998·Methods : a Companion to Methods in Enzymology·K Hunter
May 8, 1998·Methods : a Companion to Methods in Enzymology·G E Herman