PMID: 8610184Apr 2, 1996Paper

A high-resolution physical map of human chromosome 11

Proceedings of the National Academy of Sciences of the United States of America
S QinT B Shows

Abstract

The development of a highly reliable physical map with landmark sites spaced an average of 100 kbp apart has been a central goal of the Human Genome Project. We have approached the physical mapping of human chromosome 11 with this goal as a primary target. We have focused on strategies that would utilize yeast artificial chromosome (YAC) technology, thus permitting long-range coverage of hundreds of kilobases of genomic DNA, yet we sought to minimize the ambiguities inherent in the use of this technology, particularly the occurrence of chimeric genomic DNA clones. This was achieved through the development of a chromosome 11-specific YAC library from a human somatic cell hybrid line that has retained chromosome 11 as its sole human component. To maximize the efficiency of YAC contig assembly and extension, we have employed an Alu-PCR-based hybridization screening system. This system eliminates many of the more costly and time-consuming steps associated with sequence tagged site content mapping such as sequencing, primer production, and hierarchical screening, resulting in greater efficiency with increased throughput and reduced cost. Using these approaches, we have achieved YAC coverage for >90% of human chromosome 11, with an a...Continue Reading

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Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E D Green, M V Olson
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Citations

May 26, 1999·The Journal of Pathology·J KorethJ O McGee
Jan 22, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M LenerT Meo
Mar 21, 1998·Nature Genetics·V G CheungR S Spielman
May 30, 2001·European Journal of Human Genetics : EJHG·K LuS B Patel
Dec 30, 1999·Genome Research·E T PetersonD L Grady
Jul 9, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Carley D RossMichael H Fox
Feb 18, 2004·Molecular and Cellular Biology·Nicolas PineaultR Keith Humphries
Mar 1, 1997·Neuropsychology Review·D N AllenB E Seaton
Aug 1, 1997·Genome Research·C M SmithD S Gerhard
May 8, 1998·Methods : a Companion to Methods in Enzymology·G E Herman

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