A high-resolution tissue-specific proteome and phosphoproteome atlas of maize primary roots reveals functional gradients along the root axes

Plant Physiology
Caroline MarconFrank Hochholdinger

Abstract

A high-resolution proteome and phosphoproteome atlas of four maize (Zea mays) primary root tissues, the cortex, stele, meristematic zone, and elongation zone, was generated. High-performance liquid chromatography coupled with tandem mass spectrometry identified 11,552 distinct nonmodified and 2,852 phosphorylated proteins across the four root tissues. Two gradients reflecting the abundance of functional protein classes along the longitudinal root axis were observed. While the classes RNA, DNA, and protein peaked in the meristematic zone, cell wall, lipid metabolism, stress, transport, and secondary metabolism culminated in the differentiation zone. Functional specialization of tissues is underscored by six of 10 cortex-specific proteins involved in flavonoid biosynthesis. Comparison of this data set with high-resolution seed and leaf proteome studies revealed 13% (1,504/11,552) root-specific proteins. While only 23% of the 1,504 root-specific proteins accumulated in all four root tissues, 61% of all 11,552 identified proteins accumulated in all four root tissues. This suggests a much higher degree of tissue-specific functionalization of root-specific proteins. In summary, these data illustrate the remarkable plasticity of the p...Continue Reading

Citations

Aug 20, 2016·Science·Justin W WalleySteven P Briggs
Nov 10, 2018·Molecular & Cellular Proteomics : MCP·Lu-Guang JiangYan He
Mar 29, 2019·The Plant Journal : for Cell and Molecular Biology·R Glen UhrigWilhelm Gruissem
Jun 25, 2019·PLoS Biology·David Bradley, Pedro Beltrao
Jun 6, 2020·Database : the Journal of Biological Databases and Curation·Gleb MoisseyevChi Zhang
Apr 30, 2017·Biotechnology & Genetic Engineering Reviews·Monika BansalAakash Goyal
Oct 19, 2019·Journal of Experimental Botany·Ayelén GázquezGerrit T S Beemster
Mar 21, 2018·Frontiers in Plant Science·Frank HochholdingerFelix P Frey
Feb 1, 2019·Molecular & Cellular Proteomics : MCP·Lu-Guang JiangYan He
Jan 23, 2020·Journal of Proteome Research·Nagib AhsanJán A Miernyk
May 29, 2021·Biological Reviews of the Cambridge Philosophical Society·Mohammad ArefianT S Keshava Prasad

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