A high-temporal resolution technology for dynamic proteomic analysis based on 35S labeling.

PloS One
Zhao ZhangDacheng He

Abstract

As more and more research efforts have been attracted to dynamic or differential proteomics, a method with high temporal resolution and high throughput is required. In present study, a (35)S in vivo Labeling Analysis for Dynamic Proteomics (SiLAD) was designed and tested by analyzing the dynamic proteome changes in the highly synchronized A549 cells, as well as in the rat liver 2/3 partial hepatectomy surgery. The results validated that SiLAD technique, in combination with 2-Dimensional Electrophoresis, provided a highly sensitivity method to illustrate the non-disturbed endogenous proteins dynamic changes with a good temporal resolution and high signal/noise ratio. A significant number of differential proteins can be discovered or re-categorized by this technique. Another unique feature of SiLAD is its capability of quantifying the rate of protein expression, which reflects the cellular physiological turn points more effectively. Finally, the prescribed SiLAD proteome snapshot pattern could be potentially used as an exclusive symbol for characterizing each stage in well regulated biological processes.

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Citations

Jul 18, 2015·Experimental Dermatology·Shuai HaoDacheng He
Sep 16, 2011·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Yizhou WuDacheng He
Apr 16, 2015·Electrophoresis·Jinjun HeDacheng He
Jan 8, 2018·Science China. Life Sciences·Na LvDacheng He
Mar 13, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Shuai HaoChengtao Wang
Sep 27, 2018·Journal of Agricultural and Food Chemistry·Shuai HaoChengtao Wang

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Methods Mentioned

BETA
Electrophoresis
Flow Cytometry
FACS

Software Mentioned

SILAC
System
COUCTER
LabScan
SiLAD
MagicScan
ImageMaster
MatLab
SPSS13
MASCOT

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