A high-throughput and rapid method for accurate identification of emerging multidrug-resistant Candida auris

Mycoses
Ausaf AhmadOwen T Herzegh

Abstract

Candida auris is an emerging multidrug-resistant yeast associated with invasive infection in healthcare settings. Recently, C auris cases in the United States have been detected in 11 states with the majority of cases in New York, New Jersey and Illinois. Rapid and accurate identification of C auris is critical for patient care and the implementation of public health measures to control the spread of infection. Our aim was to develop and validate a rapid DNA extraction method using the Roche MagNA Pure 96 instrument and a TaqMan real-time PCR assay for reliable, high-throughput identification of C auris. We evaluated 247 patient dermal swab samples previously analysed by culture/MALDI-TOF. The diagnostic sensitivity and specificity were 93.6% and 97.2%, respectively. The assay was highly reproducible with a detection limit of 1 C auris CFU/10 μL. A receiver operating characteristic curve analysis of the real-time PCR data showed an area of 0.982 under the curve, with a CT cut-off value of ≤37.0. The turnaround time from DNA extraction to real-time PCR results was approximately 200 samples/day. In conclusion, we successfully validated a rapid and high-throughput method for accurate and reproducible identification of C auris with...Continue Reading

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Citations

Oct 9, 2019·Current Opinion in Infectious Diseases·Gabriela Corsi-Vasquez, Luis Ostrosky-Zeichner
Apr 16, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Massimo PacilliStephanie R Black
May 29, 2020·Critical Care : the Official Journal of the Critical Care Forum·Mengmeng LiJu Cao
Jul 25, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Angeliki StathiLevantia Zachariadou
Sep 5, 2019·Frontiers in Microbiology·Milena Kordalewska, David S Perlin
Dec 23, 2020·Journal of Fungi·Irene Jurado-MartínElena Eraso
Jan 31, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ahmad IbrahimFadi Bittar
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Jun 3, 2021·Journal of Fungi·Ahmad IbrahimFadi Bittar
May 13, 2021·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D Joseph SextonAnastasia P Litvintseva

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