A high-throughput continuous sample introduction interface for microfluidic chip-based capillary electrophoresis systems

Analytical Chemistry
Qun FangZhao-Lun Fang

Abstract

The development of efficient sample introduction and pretreatment systems for microfluidic chip-based analytical systems is important for their application to real-life samples. In this work, world-to-chip interfacing was achieved by a novel flow-through sampling reservoir featuring a guided overflow design. The flow-through reservoir was fabricated on a 30 x 60 x 3 mm planar glass chip of crossed-channel design used for capillary electrophoresis separations. The 20-microL sample reservoir was produced from a section of plastic pipet tip and fixed at one end of the sampling channel. Sample change was performed by pumping 80-microL samples sandwiched between air segments at approximately 0.48 mL/min flow rate through the flow-through reservoir, introduced from an access hole on the bottom side of the chip. A filter paper collar wrapped tightly around the reservoir guided the overflowing sample solution into a plastic trough surrounding the reservoir and then to waste. The performance of the system was demonstrated in the separation and determination of FITC-labeled arginine, glycine, phenylalanine, and glutamic acid with LIF detection, by continuously introducing a train of different samples through the system without electrical...Continue Reading

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Citations

Jun 21, 2005·Analytical and Bioanalytical Chemistry·S Büttgenbach, R Wilke
Aug 22, 2003·Journal of Chromatography. a·Elizabeth Smith RoddyAndrew G Ewing
Jan 19, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Huidan ZhangJin Fang
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Jul 2, 2002·Analytical Chemistry·Pierre-Alain AurouxAndreas Manz
Sep 8, 2010·Analytical Chemistry·Matthew C R Kong, Eric D Salin

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