A high-throughput in vitro model of human embryo attachment

Fertility and Sterility
Huiting HoGuiying Nie

Abstract

To develop a simple, high-throughput and widely applicable in vitro human implantation model that assesses trophoblast spheroid attachment to human uterine epithelial cells. Experimental study to establish and validate the model. Cell culture. CELL(S): Lines BeWo, RL95-2, and AN3-CA. Labeling trophoblast spheroids with green fluorescence, selecting spheroids of size similar to implanting blastocysts by use of cell strainers, and assessing spheroid attachment by use of an automated microplate reader. Establishment of a simple, reliable, and high-throughput in vitro model to study human embryo implantation. The assay enabled rapid fluorometric assessment of spheroid attachment to human endometrial epithelial cells (RL95-2 and AN3-CA) under different experimental conditions. The high-throughput assay was confirmed by conventional counting method to be highly reproducible and accurate. The described methodology provides for the first time a high-throughput assay for the study of human embryo implantation and a promising tool for screening potential inhibitors or enhancers of embryo attachment.

References

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Citations

Oct 29, 2013·Reproductive Biomedicine Online·Charlotte H E WeimarNick S Macklon
Jan 7, 2016·American Journal of Reproductive Immunology : AJRI·Hiroshi UchidaMamoru Tanaka
Sep 24, 2013·Reproductive Biomedicine Online·Charlotte H E WeimarNick S Macklon
Mar 13, 2016·Birth Defects Research. Part C, Embryo Today : Reviews·Lien M Davidson, Kevin Coward
Mar 25, 2014·Frontiers in Endocrinology·Maria Laura ZenclussenAna Claudia Zenclussen
Dec 24, 2017·Journal of Molecular Endocrinology·Kanchan GuptaAnila Dwivedi
Feb 20, 2019·Reproduction : the Official Journal of the Society for the Study of Fertility·Dongmei HeNenghui Liu
Apr 10, 2020·Future Medicinal Chemistry·Nurken Berdigaliyev, Mohamad Aljofan
Mar 10, 2017·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Xiaman HuangRuiman Li
Nov 30, 2019·Journal of Assisted Reproduction and Genetics·Jemma EvansLois A Salamonsen
Oct 14, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·R ParavatiLavinia Margarit
Jun 19, 2021·Communications Biology·Sophie BergmannThorsten E Boroviak

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