A high-throughput in vivo screening method in the mouse for identifying regulators of metastatic colonization.

Nature Protocols
Anneliese O SpeakLouise van der Weyden

Abstract

We describe a sensitive, robust, high-throughput method for quantifying the ability of metastatic tumor cells to colonize a secondary organ. Metastasis is the leading cause of death in cancer patients, and successful colonization of the secondary organ is the rate-limiting step in the metastatic process; thus, experimental methods that can be used to interrogate the key factors required for this critical step are of great importance. The experimental metastasis assay we detail here includes tail-vein injection of cancer cells into the mouse and determination of the resulting secondary organ colonization, primarily in the lung, 10 d post dosing. This assay can be used to investigate factors that regulate metastatic colonization both at the tumor-cell-intrinsic level (via manipulation of the tumor cells before injection) and at the tumor-cell-extrinsic level (such as the tissue microenvironment, via the use of genetically modified (GM) mice or agents such as antibodies or drugs). Using this method, we have robustly screened more than 950 GM mouse lines to identify novel microenvironmental regulators of metastatic colonization. The experimental details discussed here include choosing of appropriate cell numbers, handling of the ce...Continue Reading

References

Feb 7, 1990·Journal of the National Cancer Institute·I J Fidler, R Radinsky
Jan 1, 1988·Journal of Cancer Research and Clinical Oncology·G Edel
Mar 16, 1995·International Journal of Cancer. Journal International Du Cancer·P OroszD N Männel
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·S KoopA C Groom
Apr 12, 2000·Investigational New Drugs·J Nemunaitis
Jun 14, 2000·Clinical & Experimental Metastasis·S F GoldbergD R Welch
Mar 22, 2001·Radiographics : a Review Publication of the Radiological Society of North America, Inc·J B SeoM Y Kim
Sep 11, 2004·Carcinogenesis·Chand Khanna, Kent Hunter
Sep 6, 2005·Nature Genetics·Yeong-Gwan ParkKent W Hunter
Jun 29, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·J T WesselsG A Mueller
Jun 3, 2009·Psychological Methods·Patrick J Curran, Andrea M Hussong
Jan 5, 2011·International Review of Cell and Molecular Biology·Douglas R Hurst, Danny R Welch
Jan 10, 2013·PloS One·Natasha A KarpRichard F Mott
Jun 5, 2013·Neoplasia : an International Journal for Oncology Research·Mathieu Paul RoderoChristophe Combadière
Feb 1, 2015·Methods in Molecular Biology·Michael Bouvet, Robert M Hoffman
Aug 19, 2015·Cancer Research·Silja HeilmannRichard White
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Zi-Ming ZhaoJeffrey P Townsend
Mar 25, 2016·Nature Reviews. Cancer·Patricia S Steeg

❮ Previous
Next ❯

Citations

Aug 1, 2018·The Journal of Pathology·Louise van der WeydenDavid J Adams
Apr 5, 2020·G3 : Genes - Genomes - Genetics·Louise van der WeydenDavid J Adams
Oct 30, 2018·Clinical & Experimental Metastasis·Robin BoiyOlivier De Wever

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Hassan FazilatyBabak Behnam
International Journal of Cancer. Journal International Du Cancer
Wen Min LauScott L Kominsky
The West Virginia Medical Journal
Steve Brown
© 2022 Meta ULC. All rights reserved