A high throughput method to study the physiology of E2:ERα signaling in breast cancer cells.

Journal of Cellular Physiology
Stefano LeoneFilippo Acconcia

Abstract

17β-estradiol (E2) regulates diverse physiological effects including cell proliferation through the estrogen receptor α (ERα), which as a transcription factor drives gene transcription and as an extra-nuclear localized receptor triggers the membrane-dependent activation of diverse kinase cascades. E2 also modifies ERα intracellular levels via diverse intracellular mechanisms. In this way, the E2-acivated ERα integrates signaling cascades with the modulation of receptor intracellular concentration and with the induction of DNA synthesis and ultimately drives cell proliferation. In turn, E2 signaling deregulation can cause many diseases including breast cancer (BC). Recently, we performed a Western blotting (WB)-based screen to identify novel pathways affecting ERα intracellular levels and BC cell proliferation. However, because WB lacks high throughput potential, a high-content method to detect all aspects of E2:ERα signaling (nuclear and extra-nuclear receptor activity, ERα levels, E2-induced DNA synthesis) is desirable. Here, we set up a rapid way to measure E2:ERα signaling in 96-well plate format. To demonstrate its robustness, we also challenged 4OH-tamoxifen resistant (Tam-Res) BC cells with a library of anti-cancer drugs ...Continue Reading

References

Jun 6, 2000·Journal of Biomolecular Screening·J H ZhangK R Oldenburg
Dec 12, 2002·Steroids·Deborah A Lannigan
Oct 22, 2004·Molecular Biology of the Cell·Filippo AcconciaMaria Marino
Nov 16, 2004·Experimental Cell Research·Jian-Min SunJames R Davie
Aug 18, 2006·Molecular Aspects of Medicine·Paolo AscenziMaria Marino
Apr 25, 2008·Current Protocols in Immunology·Zbigniew Darzynkiewicz, Xuan Huang
May 14, 2008·Combinatorial Chemistry & High Throughput Screening·Cassia R OverkJudy L Bolton
Dec 15, 2010·Current Medicinal Chemistry·F LumachiV Camozzi
Jun 18, 2011·Endocrine Reviews·Muriel Le RomancerLaura Corbo
Jul 23, 2011·Nature Reviews. Cancer·Christoforos Thomas, Jan-Åke Gustafsson
Sep 1, 2011·Nature Reviews. Drug Discovery·Matthew G Vander Heiden
Mar 27, 2012·Molecular Endocrinology·Piergiorgio La RosaFilippo Acconcia
Mar 15, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J FerlayF Bray
Nov 5, 2013·Nature Genetics·Dan R RobinsonArul M Chinnaiyan
Dec 29, 2013·Proceedings of the National Academy of Sciences of the United States of America·Marine AdlanmeriniFrançoise Lenfant
Dec 23, 2014·International Journal of Cancer. Journal International Du Cancer·Sonja ThalerJonathan P Sleeman
Jan 16, 2016·Cell Metabolism·Natalya N Pavlova, Craig B Thompson
Mar 8, 2016·Cell Reports·James CampbellChristopher J Lord
Mar 25, 2016·Scientific Reports·Pierangela TottaFilippo Acconcia
Sep 20, 2016·Cancer Treatment Reviews·Konstantinos TryfonidisMartine Piccart
Nov 4, 2016·Nature Reviews. Molecular Cell Biology·Ellis R Levin, Stephen R Hammes
Nov 7, 2016·Molecular and Cellular Endocrinology·Filippo AcconciaMaria Marino
Nov 9, 2016·Molecular Aspects of Medicine·Paolo AscenziMaria Marino
Nov 26, 2016·Cancer Treatment Reviews·Lindsay AngusStefan Sleijfer
May 26, 2017·Physiological Reviews·Jean-Francois ArnalJohn Katzenellenbogen
Aug 24, 2017·Rheumatology and Therapy·Philipp Rein, Ruediger B Mueller

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Citations

Jan 29, 2020·Journal of Cellular Physiology·Manuela CipollettiFilippo Acconcia
May 16, 2020·International Journal of Molecular Sciences·Stefania BartoloniFilippo Acconcia
May 31, 2020·Cells·Filippo Acconcia
Mar 27, 2020·OncoTargets and Therapy·Yanfang LiuJing Yao

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