A High-Throughput Platform for Formulating and Screening Multifunctional Nanoparticles Capable of Simultaneous Delivery of Genes and Transcription Factors

Angewandte Chemie
Yang LiuHao Wang

Abstract

Simultaneous delivery of multiple genes and proteins (e.g., transcription factors; TFs) is an emerging issue surrounding therapeutic research due to their ability to regulate cellular circuitry. Current gene and protein delivery strategies, however, are based on slow batch synthesis, which is ineffective, poorly controlled, and incapable of simultaneous delivery of both genes and proteins with synergistic functions. Consequently, advances in this field have been limited to in vitro studies. Here, by integrating microfluidic technologies with a supramolecular synthetic strategy, we present a high-throughput approach for formulating and screening multifunctional supramolecular nanoparticles (MFSNPs) self-assembled from a collection of functional modules to achieve simultaneous delivery of one gene and TF with unprecedented efficiency both in vitro and in vivo. We envision that this new approach could open a new avenue for immunotherapy, stem cell reprogramming, and other therapeutic applications.

References

Oct 6, 1988·Nature·I SadowskiM Ptashne
Sep 27, 1995·Biochimica Et Biophysica Acta·C O Fágáin
Mar 19, 2002·Advanced Drug Delivery Reviews·Yoshinori Kakizawa, Kazunori Kataoka
Oct 3, 2002·Nature Reviews. Cancer·James E Darnell
Aug 24, 2006·Angewandte Chemie·Jinyi WangHsian-Rong Tseng
Apr 19, 2007·Annual Review of Biomedical Engineering·Shuming NieJonathan W Simons
Jun 10, 2008·Chemistry : a European Journal·Jun-ichi YoshidaTakeshi Yamada
Sep 2, 2008·Nature Reviews. Drug Discovery·Mark E DavisDong M Shin
Dec 2, 2008·Cell Stem Cell·Nimet Maherali, Konrad Hochedlinger
May 9, 2009·Angewandte Chemie·Hao WangHsian-Rong Tseng
Feb 6, 2010·Journal of the American Chemical Society·Partha GhoshVincent M Rotello
Feb 25, 2010·Chemical Society Reviews·Samuel Marre, Klavs F Jensen
Mar 4, 2010·Chemical Communications : Chem Comm·Hao WangHsian-Rong Tseng
Mar 9, 2010·Nano Today·Wei-Yu LinHsian-Rong Tseng
Dec 14, 2011·Angewandte Chemie·Nathan P GabrielsonJianjun Cheng
Jun 22, 2013·Chemical Communications : Chem Comm·Carmen Stoffelen, Jurriaan Huskens
Apr 29, 2015·Nature Materials·Kunwoo LeeNiren Murthy

❮ Previous
Next ❯

Citations

Jul 19, 2016·International Journal of Pharmaceutics·Yujie LiWeiping Zhu
Jun 29, 2017·ACS Applied Materials & Interfaces·Fangfang LiLichen Yin
Sep 16, 2017·Lab on a Chip·Yong Liu, Xingyu Jiang
Sep 15, 2018·Nanotechnology·Ziyi HePeng Li
Jan 12, 2019·Chemical Communications : Chem Comm·Yu-Hui ZhangYu Liu
Jan 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Raquel Mejia-ArizaJurriaan Huskens
May 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Huijun ZhangDaoben Hua
Dec 18, 2019·International Journal of Pharmaceutics·Sai LuJinliang Peng
Dec 19, 2020·Nanomaterials·Peipei ZhouJianhua Zhou
Dec 27, 2017·Advanced Drug Delivery Reviews·Xuanyu Li, Xingyu Jiang
Jul 17, 2018·ACS Applied Materials & Interfaces·Xu-Man ChenYu Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.