A high-throughput screen to identify novel compounds to promote neurite outgrowth

Journal of Neuroscience Methods
Jennifer W J WongTimothy P O'Connor

Abstract

Following spinal cord injury, a variety of inhibitory molecules hinder the success of axon regeneration. The motile tip of the axon, the growth cone, shares a similar cytoskeletal array as a migrating cell, and in general the cytoskeleton is regulated by a conserved set of signaling pathways that act downstream of guidance cue and growth factor receptors. We exploit these similarities by using migrating cells as a model system to screen for extracts that promote axon outgrowth. The screen is a high-throughput wound-healing assay performed by a 96-pin tool Biogrid robot where positive candidates are identified as extracts that stimulate complete wound healing. Testing of positive candidates on chick DRG explants has lead to the identification of extracts that promote neurite outgrowth on permissive and inhibitory substrates. Extracts can be fractionated to purity, identifying novel compounds that promote neurite outgrowth on inhibitory substrates.

References

Oct 1, 1973·Journal of Neurochemistry·W T Norton, S E Poduslo
Feb 17, 2001·Current Opinion in Neurobiology·B J Dickson
Aug 30, 2001·Experimental Cell Research·N SogaK A Hruska
Feb 28, 2002·Molecular and Cellular Neurosciences·Matt S RamerStephen B McMahon
Apr 12, 2002·Nature·Elizabeth J BradburyStephen B McMahon
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Liqun Luo
Nov 5, 2003·Journal of Neurobiology·Gianluca Gallo, Paul C Letourneau
Jan 22, 2004·Nature Reviews. Neuroscience·Jerry Silver, Jared H Miller
Aug 13, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xiquan LiangMarilyn D Resh
Nov 19, 2004·The European Journal of Neuroscience·V E MacDermidM S Ramer
Nov 19, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shuxin LiStephen M Strittmatter
Sep 10, 2005·Journal of Neurochemistry·Markus HöltjeGudrun Ahnert-Hilger
Jul 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lynda J S YangRonald L Schnaar
Jul 22, 2006·Nature Reviews. Neuroscience·Glenn Yiu, Zhigang He
Oct 6, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tina ThurnherrJoão B Relvas

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Citations

Oct 18, 2008·Experimental Neurology·Jennifer W J WongTimothy P O'Connor

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