A high-throughput strategy for screening of bacterial artificial chromosome libraries and anchoring of clones on a genetic map constructed with single nucleotide polymorphisms.

BMC Genomics
Ming-Cheng LuoJ Dvorák

Abstract

Current techniques of screening bacterial artificial chromosome (BAC) libraries for molecular markers during the construction of physical maps are slow, laborious and often assign multiple BAC contigs to a single locus on a genetic map. These limitations are the principal impediment in the construction of physical maps of large eukaryotic genomes. It is hypothesized that this impediment can be overcome by screening multidimensional pools of BAC clones using the highly parallel Illumina GoldenGate assay. To test the efficacy of the Golden Gate assay in BAC library screening, multidimensional pools involving 302976 Aegilops tauschii BAC clones were genotyped for the presence/absence of specific gene sequences with multiplexed Illumina GoldenGate oligonucleotide assays previously used to place single nucleotide polymorphisms on an Ae. tauschii genetic map. Of 1384 allele-informative oligonucleotide assays, 87.6% successfully clustered BAC pools into those positive for a BAC clone harboring a specific gene locus and those negative for it. The location of the positive BAC clones within contigs assembled from 199190 fingerprinted Ae. tauschii BAC clones was used to evaluate the precision of anchoring of BAC clones and contigs on the ...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H ShizuyaM Simon
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S Brenner, K J Livak
Mar 1, 1995·Genomics·W J BrunoD C Torney
Nov 21, 1997·Computer Applications in the Biosciences : CABIOS·C SoderlundR Mott
Nov 15, 2000·Genome Research·C SoderlundL French
Jul 22, 2004·Nature Reviews. Genetics·Blake C MeyersMichele Morgante
Sep 2, 2004·Cold Spring Harbor Symposia on Quantitative Biology·P Y Kwok, M Xiao
Sep 2, 2004·Cold Spring Harbor Symposia on Quantitative Biology·J B FanM S Chee
Oct 5, 2006·Bioinformatics·Frank M YouOlin D Anderson
Mar 22, 2008·Genome Génome / Conseil National De Recherches Canada·Randy C ShoemakerScott Jackson

❮ Previous
Next ❯

Citations

Apr 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Ming-Cheng LuoJan Dvorak
Apr 14, 2012·Journal of Biomedicine & Biotechnology·Ruvini Ariyadasa, Nils Stein
Dec 3, 2014·International Journal of Molecular Sciences·Wenjin CaoHaiyan Jia
Apr 19, 2015·BMC Plant Biology·Kateřina CvikováJan Bartoš
Apr 14, 2011·BioTechniques·Hui LiuPeter E Hedley

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping
PCR
PCRs
Assay

Software Mentioned

GenoProfiler
Beadstudio

Related Concepts

Related Feeds

Artificial Chromosomes

Artificial chromosomes are genetically engineered chromosomes derived from the DNA of a species. Discover the latest research on artificial chromosomes here.