A high-throughput UPC2 -MS/MS method for the separation and quantification of C19 and C21 steroids and their C11-oxy steroid metabolites in the classical, alternative, backdoor and 11OHA4 steroid pathways

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Therina du ToitAmanda C Swart

Abstract

In the present study an ultra-performance convergence chromatography tandem mass spectrometry (UPC2-MS/MS) analytical method was developed and validated for the determination of 17 C19and 14 C21steroids, including C11-oxy C19and C11-oxy C21steroids. The limit of detection and limit of quantification ranged from 0.01 to 10 ng/mL and from 0.01 to 20 ng/mL, respectively, and the method shows the recovery, matrix effect and process efficiency of steroids isolated from a serum matrix to be within acceptable limits. Good accuracy, repeatability and reproducibility were also shown and the method provided excellent sensitivity and selectivity as stereoisomers and regioisomers were also resolved and quantified accurately. Clinical conditions such as congenital adrenal hyperplasia, polycystic ovary syndrome in females and disorders of sex development in neonates and in children, amongst others, are characterized by abnormal steroid levels. Steroid profiling is essential to accurately diagnose steroid levels in the above settings as well as in androgen excess or deficiency in adrenal-linked endocrine diseases. Our method, separating C19and C21steroids in a single chromatographic step, offers a reduced sample turnover rate in the clinical ...Continue Reading

Citations

Mar 21, 2019·Current Opinion in Endocrinology, Diabetes, and Obesity·Flaminia Fanelli, Guido Di Dalmazi
May 5, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing GuoBei Fan
Apr 30, 2019·Frontiers in Endocrinology·Amanda C SwartStephen L Atkin
Feb 29, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Nico LübckerRobert P Millar
Jul 23, 2020·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Qianqian LiShaofeng Rong

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