A high-yield chromatographic method for the purification of rat fibroblast interferon to a high specific activity

Journal of Interferon Research
P Kaplan, S L Abreu

Abstract

Rat fibroblast interferon (RfIFN), produced in serum-free media, has been purified 2,000-fold to 88% purity by a simple method that involves only two chromatographic steps: controlled pore glass and phenyl agarose chromatography. Over 70% of the initial activity was recovered. Gel electrophoresis (SDS-PAGE) and silver staining of the purified RfIFN revealed two major bands of RfIFN activity corresponding to Mr of 26,000 and 22,500 daltons, respectively. A very small amount of RfIFN was also recovered from a region of the gel corresponding to a Mr of 18,500. The average specific activity of the purified material was 3.7 X 10(8) U/mg. From these results it can be calculated that the specific activity of homogenous RfIFN is 4.0 X 10(8) U/mg.

References

Jan 1, 1979·Preparative Biochemistry·E Bollin, E Sulkowski
Jan 1, 1979·Archives of Virology·L S Lin, S L Abreu
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M RubinsteinS Pestka
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K C ZoonC B Anfinsen
Dec 1, 1976·The Journal of General Virology·V G EdyP De Somer
Feb 28, 1966·Biochimica Et Biophysica Acta·E Schonne
Jan 1, 1980·Preparative Biochemistry·A J MikulskiE Sulkowski
Jan 1, 1980·Scandinavian Journal of Immunology·K Berg, I Heron
Feb 1, 1981·Archives of Biochemistry and Biophysics·H J FriesenS Pestka
Feb 1, 1981·The Journal of General Virology·E Bollin, E Sulkowski
Jan 1, 1981·Preparative Biochemistry·K C Chadha, E Sulkowski
Jan 15, 1982·International Journal of Cancer. Journal International Du Cancer·J AbbF Deinhardt
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·Y K YipJ Vilcek
Apr 1, 1984·The Journal of General Virology·T R WinshipG D Hsiung
Jan 1, 1980·The Journal of General Virology·H SchellekensW Weimar

Related Concepts

Chromatography
Ion-Exchange Chromatography Procedure
SDS-PAGE
Fibroblasts
Glass (Substance)
Interferon Type I
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