PMID: 6979540Jul 25, 1982Paper

A high yield purification procedure for alpha and beta chains of HLA-DR antigens.

The Journal of Biological Chemistry
L E Walker, R A Reisfeld

Abstract

A procedure is described for purifying to homogeneity the alpha and beta chains of HLA-DR antigen from the homozygous human lymphoid cell line LG-2. The procedure involves a combination of lectin affinity as well as classical and high pressure liquid chromatography. From 100 g of packed cells, 8 mg (235 nmol) of alpha chain and 7 mg (205 nmol) of beta chain were isolated.

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