A highly efficient maize nucellus protoplast system for transient gene expression and studying programmed cell death-related processes

Plant Cell Reports
Jiang ChenYubi Huang

Abstract

Conditions for the isolation and transfection of maize nucellus protoplasts were established. We demonstrated its utilization for protein expression, localization, protein-protein interaction, and the investigation of PCD-related processes. Plant protoplasts are an important and versatile cell system that is widely used in the analysis of gene characterization and diverse signaling pathways. Programmed cell death (PCD) occurs throughout the life of plants from embryogenesis to fertilization. The maize nucellus undergoes typical PCD during development of the embryo sac. The nucellus protoplast shows potential for use in research of PCD-related processes. No studies have reported previously the isolation and transfection of nucellus protoplasts. In this study, conditions for the isolation and transfection of maize nucellus protoplasts were established. The maize protoplast system can be used for protein expression, localization, and protein-protein interaction. We applied this system to investigate PCD-related processes. Quantitative real-time PCR analysis revealed that transient expression of MADS29 in the maize nucellus protoplast increases Cys-protease gene transcript level. In addition, β-glucuronidase and luciferase activity...Continue Reading

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Citations

Jun 15, 2016·Annual Review of Cell and Developmental Biology·Anna DanevaMoritz K Nowack
Oct 30, 2016·Molecules and Cells·Kyunghyuk ParkYeonhee Choi
Feb 23, 2019·Journal of Experimental Botany·Rafael Andrade BuonoMoritz K Nowack
Oct 6, 2016·Journal of Experimental Botany·Gwyneth C Ingram
Dec 12, 2020·Plant Physiology and Biochemistry : PPB·Yukun WangFei Ren
Aug 28, 2021·International Journal of Molecular Sciences·Safia SaadaSinéad Drea

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