A highly efficient method for generation of therapeutic quality human pluripotent stem cells by using naive induced pluripotent stem cells nucleus for nuclear transfer

SAGE Open Medicine
Madhusudana Girija Sanal

Abstract

Even after several years since the discovery of human embryonic stem cells and induced pluripotent stem cells (iPSC), we are still unable to make any significant therapeutic benefits out of them such as cell therapy or generation of organs for transplantation. Recent success in somatic cell nuclear transfer (SCNT) made it possible to generate diploid embryonic stem cells, which opens up the way to make high-quality pluripotent stem cells. However, the process is highly inefficient and hence expensive compared to the generation of iPSC. Even with the latest SCNT technology, we are not sure whether one can make therapeutic quality pluripotent stem cell from any patient's somatic cells or by using oocytes from any donor. Combining iPSC technology with SCNT, that is, by using the nucleus of the candidate somatic cell which got reprogrammed to pluripotent state instead that of the unmodified nucleus of the candidate somatic cell, would boost the efficiency of the technique, and we would be able to generate therapeutic quality pluripotent stem cells. Induced pluripotent stem cell nuclear transfer (iPSCNT) combines the efficiency of iPSC generation with the speed and natural reprogramming environment of SCNT. The new technique may be ...Continue Reading

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Citations

Jan 27, 2015·Scientific Reports·Paolo PiattiAlexandra Lusser
Jun 16, 2015·World Journal of Gastroenterology : WJG·Madhusudana Girija Sanal
Jun 7, 2019·Stem Cell Research·Kathryn M HeadleyNathaniel A Hathaway

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Methods Mentioned

BETA
transgenic
transfection

Software Mentioned

iPSCNT

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