A highly efficient plant regeneration system through multiple shoot differentiation from commercial cultivars of barley (Hordeum vulgare L.) using meristematic shoot segments excised from germinated mature embryos

Plant Cell Reports
Vijendra K SharmaJutta Schulze

Abstract

A fast and highly efficient short-term in vitro regeneration system was developed for barley (Hordeum vulgare L.) based on readily available explants. Clumps of multiple shoots and buds suitable for transformation were obtained 9-10 weeks after culture initiation from model and current commercial cultivars. Meristematic shoot segments (MSSs) excised from mature embryo-derived seedlings and subsequently cultured on MS-based medium containing 2 mg/l Picloram and 3 mg/l thidiazuron (TDZ) differentiated up to ten multiple shoots after 3-4 weeks with no or very little callus formation. Sectors of the already multiplied shoot clumps were further multiplied on proliferation-maintenance medium containing 2 mg/l Picloram and 2.5 mg/l TDZ. Biweekly subcultures resulted in a continuous process of multiplication of these highly differentiating green sectors without any loss of morphogenic potential. The differentiated small shoots and shoot buds gave rise to normal shoots on medium with 0.1 mg/l Picloram and 1 mg/l TDZ. After rooting on basal medium with 0.5 mg/l or 1 mg/l IBA the plants were transferred to soil and showed normal growth and fertility compared to the seed-grown plants. All of the genotypes tested formed multiple shoots. The...Continue Reading

Citations

Dec 2, 2006·Journal of Plant Research·Nimnara YookongkaewJarunya Narangajavana
Oct 2, 2013·Trends in Plant Science·Gerald N De La FuenteThomas Lübberstedt
May 1, 2007·Pakistan Journal of Biological Sciences : PJBS·Esmaeil EbrahimieManijeh Mohammadie-Dehcheshmeh
Jan 7, 2016·Plant Physiology and Biochemistry : PPB·Hiroshi HisanoKazuhiro Sato
Apr 30, 2011·Journal of Zhejiang University. Science. B·Yong HanGuo-ping Zhang

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