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A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae

Metabolic Engineering

Nov 8, 2015

Shuobo ShiHuimin Zhao

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PubMed

Abstract

Despite recent advances in genome editing capabilities for the model organism Saccharomyces cerevisiae, the chromosomal integration of large biochemical pathways for stable industrial production remains challenging. In this work, we developed a simple platform for high-efficiency, singl...read more

Mentioned in this Paper

Saccharomyces cerevisiae Proteins
Real-Time Polymerase Chain Reaction
LYP1
Flow Cytometry
Biochemical Pathway
Genetically Engineered Mouse
Gene Dosage
Oculocerebrorenal Syndrome
Genome
CRISPR-Cas Systems
2
285
Paper Details
References
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A highly efficient single-step, markerless strategy for multi-copy chromosomal integration of large biochemical pathways in Saccharomyces cerevisiae

Metabolic Engineering

Nov 8, 2015

Shuobo ShiHuimin Zhao

PMID: 26546089

DOI: 10.1016/j.ymben.2015.10.011

Abstract

Despite recent advances in genome editing capabilities for the model organism Saccharomyces cerevisiae, the chromosomal integration of large biochemical pathways for stable industrial production remains challenging. In this work, we developed a simple platform for high-efficiency, singl...read more

Mentioned in this Paper

Saccharomyces cerevisiae Proteins
Real-Time Polymerase Chain Reaction
LYP1
Flow Cytometry
Biochemical Pathway
Genetically Engineered Mouse
Gene Dosage
Oculocerebrorenal Syndrome
Genome
CRISPR-Cas Systems
2
285

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CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

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SSFinder: high throughput CRISPR-Cas target sites prediction tool

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Paper Details
References
  • References36
  • Citations28
1234
  • References36
  • Citations28
123

Download from

Publisher
PubMed
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