A highly expressing Tet-inducible cell line recapitulates in situ developmental changes in prestin's Boltzmann characteristics and reveals early maturational events.

American Journal of Physiology. Cell Physiology
Shumin BianDhasakumar Navaratnam

Abstract

Prestin is the motor protein within the lateral membrane of outer hair cells (OHCs), and it is required for mammalian cochlear amplification. Expression of prestin precedes the onset of hearing in mice, and it has been suggested that prestin undergoes a functional maturation within the membrane coincident with the onset of hearing. We have developed a tetracycline-inducible prestin-expressing cell line that we have used to model prestin's functional maturation. We used prestin's voltage-dependent nonlinear charge movement (or nonlinear capacitance) as a test of function and correlated it to biochemical measures of prestin expressed on the cell surface. An initial stage of slow growth in charge density is accompanied by a rapid increase in our estimate of charge carried by an individual motor. A rapid growth in charge density follows and strongly correlates with an increasing ratio between an apparently larger and smaller monomer, suggesting that the latter exerts a dominant-negative effect on function. Finally, there is a gradual depolarizing shift in the voltage of peak capacitance, similar to that observed in developing OHCs. This inducible system offers many opportunities for detailed studies of prestin.

References

Mar 1, 1987·Brain Research·B J BlatchleyJ R Coleman
Jan 11, 1985·Science·W E BrownellY de Ribaupierre
Aug 23, 2005·Biophysical Journal·Dhasakumar NavaratnamJoseph Santos-Sacchi
Apr 14, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joseph Santos-SacchiAlfred L Nuttall
May 10, 2006·The Journal of Biological Chemistry·Jing ZhengMaryAnn Cheatham
Feb 27, 2007·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Angela K SturmFred A Pereira
Apr 7, 2007·The Journal of Membrane Biology·Takahisa AbeHideichi Shinkawa
Nov 9, 2007·Journal of Neurophysiology·Lei SongEdward J Walsh
Dec 13, 2007·The Journal of Biological Chemistry·Silvia Detro-DassenChristoph Fahlke
Jan 30, 2008·Protein Expression and Purification·Elisa PasqualettoRoberto Battistutta

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Citations

Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lei Song, Joseph Santos-Sacchi
Apr 20, 2017·Scientific Reports·Jun-Ping BaiDhasakumar Navaratnam
Feb 9, 2016·Hearing Research·Channy ParkFederico Kalinec
May 10, 2020·The Journal of General Physiology·Brenda FarrellWilliam E Brownell

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