A highly flexible tRNA acylation method for non-natural polypeptide synthesis

Nature Methods
Hiroshi MurakamiHiroaki Suga

Abstract

Here we describe a de novo tRNA acylation system, the flexizyme (Fx) system, for the preparation of acyl tRNAs with nearly unlimited selection of amino and hydroxy acids and tRNAs. The combination of the Fx system with an appropriate cell-free translation system allows us to readily perform mRNA-encoded synthesis of proteins and short polypeptides involving multiple non-natural amino acids.

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Citations

Jun 30, 2011·Accounts of Chemical Research·Jumpei MorimotoHiroaki Suga
Jun 28, 2012·ACS Synthetic Biology·Harris H WangGeorge M Church
Nov 17, 2009·Nature Chemical Biology·Philip R EffraimVirginia W Cornish
Nov 17, 2009·Nature Chemical Biology·Takashi KawakamiHiroaki Suga
Jan 22, 2008·Nature Methods·Mingyue HeMichael J Taussig
Jun 4, 2011·Nature Protocols·Yuki GotoHiroaki Suga
Jun 9, 2009·Chemical Communications : Chem Comm·Yuki GotoHiroaki Suga
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Karin ÖjemalmGunnar von Heijne
May 25, 2010·Journal of Biochemistry·Koichiro KodamaShigeyuki Yokoyama
Aug 28, 2012·Journal of Biochemistry·Hiroaki Imataka
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Sep 17, 2009·Protein Engineering, Design & Selection : PEDS·Bill EldridgeChristopher G Ullman
Sep 21, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Hiroaki SugaNaohiro Terasaka
Apr 30, 2008·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Taek Jin Kang, Hiroaki Suga
Jun 3, 2008·RNA·Yuki GotoHiroaki Suga
Sep 13, 2007·International Journal of Biological Sciences·Shingo UenoYuzuru Husimi
Mar 4, 2014·Annual Review of Biochemistry·Toby PassiouraHiroaki Suga
Feb 6, 2014·Applied Microbiology and Biotechnology·Darko BalkeSabine Müller
Jun 9, 2014·Nature Chemical Biology·Naohiro TerasakaHiroaki Suga
Jun 16, 2014·Nature Chemical Biology·Jochen SpiegelHerbert Waldmann
Nov 6, 2010·Journal of Biomedicine & Biotechnology·Brian R FritzMichael C Jewett
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Sep 4, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Christopher J HipolitoHiroaki Suga
Mar 20, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kenichiro ItoHiroaki Suga
Jan 26, 2016·Journal of the American Chemical Society·Tomoshige FujinoHiroshi Murakami
Apr 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Michael T EnglanderVirginia W Cornish
Apr 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ziwei LiuKarin Musier-Forsyth
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