A highly integrated DNA nanomachine operating in living cells powered by an endogenous stimulus

Chemical Science
Pei-Qiang MaBang-Ce Ye

Abstract

Synthetic molecular machines have received increasing attention because of their great ability to mimic natural biological motors and create novel modes of motion. However, very few examples have been implemented with real autonomous movement inside living cells, due to the challenges of the driving force and highly integrated system design. In this work, we report an elegant, highly integrated DNA nanomachine that can be powered by endogenous ATP molecules and autonomously operated inside living cells without any auxiliary additives. It assembles all components on a single gold nanoparticle (AuNP) including a hairpin-locked swing arm encoding a start triggered by an intracellular target molecule and a two-stranded DNA track responding to the motion of the swing arm. When the intracellular target activates the nanomachine via the unlocking swing arm, the machine autonomously and progressively operates on the established DNA track via intramolecular toehold-mediated strand migration and internal ATP binding. This paper also demonstrates the machine's bioanalytical application for specific microRNA (miRNA) imaging in living cells.

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Citations

Feb 2, 2019·Angewandte Chemie·Zhihe QingRonghua Yang
Jul 5, 2019·Advanced Materials·Devleena SamantaChad A Mirkin
Dec 14, 2019·Chemical Communications : Chem Comm·Namin HuXia Chu
Feb 27, 2020·Chemical Communications : Chem Comm·Chang LiuXia Chu
May 12, 2019·Nucleic Acids Research·Shungo KoboriYohei Yokobayashi
Dec 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dinggeng HeHung-Wing Li
Oct 31, 2018·Biosensors·Marta Maria Pereira da Silva Neves, Daniel Martín-Yerga
May 8, 2019·Chemical Communications : Chem Comm·Xiaowen XuWei Jiang
Aug 14, 2019·Chemical Society Reviews·Wenjing WangJun-Jie Zhu
Jul 28, 2020·Advanced Science·Yaqin HuYanfei Liu
Jan 19, 2021·Biosensors & Bioelectronics·Jie FangGuoming Xie
Feb 8, 2020·Journal of the American Chemical Society·Pei-Qiang MaBang-Ce Ye
Mar 13, 2019·Analytical Chemistry·Jing XueYongxi Zhao
Jan 6, 2022·Chemical Society Reviews·Chen WangItamar Willner
Jan 11, 2022·Langmuir : the ACS Journal of Surfaces and Colloids·Wukun ZhongChunhua Lu

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Methods Mentioned

BETA
electrophoresis
transfection

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