PMID: 8960100Nov 29, 1996Paper

A highly precise reporter gene bioassay for type I interferon

Journal of Immunological Methods
U CanosiG Galli

Abstract

We describe the setting up and validation of a reporter gene assay for type I IFN based on monkey Vero cells transfected with pMx-Luc, a plasmid carrying the luciferase gene under the control of the type I IFN inducible mouse Mx1 promoter. Vero cells were stably transfected with pMx-Luc and clone 3-143/5 was selected on the basis of luciferase inducibility by IFN-beta. A linear dose-response relationship was found between 1 and 16 IU/ml IFN-beta. The assay was shown to be specific for IFN-alpha and -beta as no effect by a number of other cytokines including IFN-gamma could be detected. In order to render the IFN-beta reporter gene assay protocol more suitable for routine assays, a 3 x 3 balanced parallel line assay design was applied using a 96-well luminometer for luminescence measurement. The assay was shown to be precise with a coefficient of variation of less than 9%. This assay is characterized by high precision coupled to high efficiency, as reflected by a very short assay duration (1 day), when compared to the classical cytopathic effect assays for IFNs and the previously published IFN reporter gene assay based on growth hormone measurement (Lleonart, R., Näf, D., Browning, H. and Weissmann, C. (1990) A novel, quantitati...Continue Reading

References

Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J PriceC Cepko
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M O DiazJ D Rowley
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R A JeffersonD Hirsh
Jul 1, 1984·Journal of Biological Standardization·D J Giard, R J Fleischaker

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Citations

Feb 28, 2001·Journal of Immunological Methods·M D FrayB Charleston
Feb 28, 2002·Journal of Immunological Methods·Anthony Meager
Feb 12, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·X Van OstadeJ Tavernier
Jan 7, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J G FilesE Pungor
Aug 26, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·U HammerlingL Sjödin
Dec 9, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Daniela KugelPeter Staeheli
Mar 25, 2006·Uirusu·Subaru OgisoEiichi Honda
May 3, 2012·Journal of Immunological Methods·María de Los Milagros BürgiMariela Bollati-Fogolín
May 7, 2008·Molecular Immunology·Rosario CastroChristopher J Secombes
Feb 26, 2008·Journal of Immunological Methods·V SmilovićV Menart
Mar 23, 2006·Fish & Shellfish Immunology·Ei Lin OoiTakashi Aoki
Oct 8, 2005·Journal of Immunological Methods·Mariela Bollati-Fogolín, Werner Müller
Mar 16, 2011·Journal of Biomedicine & Biotechnology·Louise LarocqueXuguang Li
Mar 22, 2018·Nature Reviews. Rheumatology·Theresa L Wampler Muskardin, Timothy B Niewold
Feb 23, 2018·Biomedical Research·Noriyuki TojiKazuyoshi Hashizume
Jun 13, 2018·Journal of Immunological Methods·Phyllis A Rees, R Joel Lowy

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