A highly secure method for rearing Aedes aegypti mosquitoes

Tropical Medicine and Health
Manabu Ote, Hirotaka Kanuka

Abstract

Vector-borne infectious diseases are caused by pathogenic microorganisms transmitted mainly by blood-sucking arthropod vectors. In laboratories, the handling of insects carrying human pathogens requires extra caution because of safety concerns over their escape risk. Based on standard insect containment practices, there have been cases where costly enhancements were required to definitely protect laboratory workers and neighbors from potential infection through mosquito bites. Here, we developed a mosquito rearing method that provides a reliable and cost-effective means to securely contain pathogen-infected females of the yellow fever mosquito Aedes aegypti. To debilitate the motility of A. aegypti females, mosquitoes were rendered completely flightless by ablation of either wing. The "single-winged" mosquitoes exhibited a severe defect in flying ability and were incubated in a container with inside surfaces covered with a net stretched to approximately 1-mm mesh, which helped the mosquitoes hold on and climb up the wall. In this container, flightless females consistently showed similar blood feeding and egg laying activities to intact females. Eighty-five percent of the flightless mosquitoes survived at 1 week after wing ablat...Continue Reading

References

Jan 1, 1979·The American Journal of Tropical Medicine and Hygiene·T H AitkenL Rosen
Nov 1, 1992·The American Journal of Tropical Medicine and Hygiene·S C WeaverT W Scott
Sep 1, 1971·The American Journal of Tropical Medicine and Hygiene·J R Larsen, R F Ashley
May 25, 1999·Journal of Medical Entomology·D V CanyonR Muller
Aug 12, 2003·Vector Borne and Zoonotic Diseases·UNKNOWN American Committee of Medical Entomology, UNKNOWN American Society of Tropical Medicine and Hygiene
Dec 16, 2009·Malaria Journal·Michelle E H HelinskiBart G J Knols
Oct 12, 2010·PLoS Neglected Tropical Diseases·Chonggang XuAlun L Lloyd
Dec 15, 2010·Vector Borne and Zoonotic Diseases·Zoe L LyskiDoria F Bowers
Dec 12, 2012·PloS One·Miranda Chan, Michael A Johansson
Jul 5, 2013·PLoS Neglected Tropical Diseases·Nils Benjamin TjadenCarl Beierkuhnlein

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

R

Related Concepts

Related Papers

Bulletin of the World Health Organization
F L SOPER
Boletín de la Oficina Sanitaria Panamericana
F L SOPER
Immunological Reviews
Stefanie Steinert, Elena A Levashina
© 2022 Meta ULC. All rights reserved