A highly selective and sensitive Zn2+ fluorescent sensor based on zinc finger-like peptide and its application in cell imaging.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Shuaibing YuYing-Wu Lin

Abstract

Developing new chemosensors for detection of Zn2+ has attracted great attentions because of the important roles of Zn2+ in biological systems, and it will produce toxic effects with an excessive intake of zinc ion. Metalloproteins are often used as an effective template for the design and development of peptide-based fluorescent sensors. In this study, we designed a new and simple ratiometric fluorescent sensor for Zn2+, which was based on a zinc finger-like peptide and labeled with a dansyl group, i.e., Dansyl-His-Gln-Arg-Thr-His-Trp-NH2 (D-P6), by using solid phase peptide synthesis (SPPS). The dimeric peptide has a high affinity for Zn2+ overothermetalions, as indicated by spectroscopic studies, as well as molecular modeling. Remarkably, the sensor exhibited a highly selective and sensitive ratiometric fluorescent response to Zn2+ by fluorescent resonance energy transfer effect between tryptophan residue and fluorophore dansyl group, with a very low detection limit of 33 nM in aqueous solution. Furthermore, the sensor displayed a very low biotoxicity, which allows successful detection of Zn2+ in living HeLa cells. We believe that the new sensor may have potential applications in biological science.

References

Mar 1, 1990·International Journal of Peptide and Protein Research·G B Fields, R L Noble
Feb 1, 1996·Journal of Molecular Graphics·W HumphreyK Schulten
Nov 1, 1996·Nature Structural Biology·C A Kim, J M Berg
Feb 8, 2002·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·G K Andrews
Mar 26, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shawn C Burdette, Stephen J Lippard
Apr 23, 2003·Analytical Chemistry·Yujun ZhengRoger M Leblanc
Jun 11, 2004·Annual Review of Nutrition·Christa Fischer Walker, Robert E Black
Jun 11, 2004·Annual Review of Nutrition·Pamela J Fraker, Louis E King
May 14, 2005·Nature Reviews. Neuroscience·Christopher J FredericksonAshley I Bush
Sep 30, 2005·Environmental Science & Technology·Andreas VoegelinRuben Kretzschmar
May 18, 2006·Journal of the American Chemical Society·Kazuki KiyoseTetsuo Nagano
Apr 19, 2007·Chemistry, an Asian Journal·Priya CarolAyyappanpillai Ajayaghosh
Oct 2, 2007·Journal of the American Chemical Society·Amit R ReddiBrian R Gibney
Oct 12, 2007·Journal of the American Chemical Society·Kensuke KomatsuTetsuo Nagano
Apr 23, 2008·Chemical Reviews·Emily L QueChristopher J Chang
Jul 26, 2008·Chemical Reviews·Elizabeth M Nolan, Stephen J Lippard
Sep 9, 2008·Journal of the American Chemical Society·Sergey Malashikhin, Nathaniel S Finney
Apr 9, 2009·Angewandte Chemie·Shin MizukamiKazuya Kikuchi
Jun 3, 2009·Journal of the American Chemical Society·Ruslan GuliyevEngin U Akkaya
Mar 24, 2010·Chemical Communications : Chem Comm·Chang Su LimBong Rae Cho
Jan 21, 2011·Current Opinion in Biotechnology·Kouichi Kuroda, Mitsuyoshi Ueda
Feb 14, 2012·Chemical Communications : Chem Comm·Joung-Min KimKeun-Hyeung Lee
May 15, 2012·Bioorganic & Medicinal Chemistry Letters·Yan LiYangzhong Liu
Nov 20, 2014·Chemical Society Reviews·Hao ZhuXiaojun Peng
Jul 11, 2018·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Peng WangBo Chen
May 11, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Xuliang PangLianzhi Li
May 7, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Peng WangYu Tang
Jul 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Peng WangYu Tang
Nov 21, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Giuliana DonadioEugenio Notomista

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Yi Chen, De X Zeng
Current Opinion in Chemical Biology
Kazuya KikuchiTetsuo Nagano
Bioorganic & Medicinal Chemistry Letters
Bishnu Prasad JoshiKeun-Hyeung Lee
© 2021 Meta ULC. All rights reserved