A Highly Selective Electrochemical DNA-Based Sensor That Employs Steric Hindrance Effects to Detect Proteins Directly in Whole Blood

Journal of the American Chemical Society
Sahar Sadat MahshidAlexis Vallée-Bélisle

Abstract

Here we describe a highly selective DNA-based electrochemical sensor that utilizes steric hindrance effects to signal the presence of large macromolecules in a single-step procedure. We first show that a large macromolecule, such as a protein, when bound to a signaling DNA strand generates steric hindrance effects, which limits the ability of this DNA to hybridize to a surface-attached complementary strand. We demonstrate that the efficiency of hybridization of this signaling DNA is inversely correlated with the size of the molecule attached to it, following a semilogarithmic relationship. Using this steric hindrance hybridization assay in an electrochemical format (eSHHA), we demonstrate the multiplexed, quantitative, one-step detection of various macromolecules in the low nanomolar range, in <10 min directly in whole blood. We discuss the potential applications of this novel signaling mechanism in the field of point-of-care diagnostic sensors.

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Citations

Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley
Aug 12, 2016·Journal of the American Chemical Society·Jagotamoy DasShana O Kelley
Dec 14, 2016·Analytical Chemistry·Samiksha NayakSamuel K Sia
Jan 13, 2017·Nanomedicine·Arnaud Desrosiers, Alexis Vallée-Bélisle
Feb 9, 2017·Journal of the American Chemical Society·Allen L NgYuHuang Wang
Feb 13, 2016·Advanced Healthcare Materials·Tina Saberi SafaeiShana O Kelley
Jul 25, 2019·Angewandte Chemie·Jagotamoy Das, Shana O Kelley
Feb 22, 2018·Nanoscale·Scott G HarrounAlexis Vallée-Bélisle
Apr 14, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Youngjun Song
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Katarína Nemčeková, Ján Labuda
Mar 29, 2019·Bioelectrochemistry·Jeyaraman Shankara Narayanan, Gymama Slaughter
Jan 10, 2018·Journal of the American Chemical Society·Alessandro PorchettaFrancesco Ricci
Mar 26, 2019·Analytical Chemistry·Sahar Sadat MahshidShana O Kelley
Dec 13, 2019·ACS Sensors·Yifan DaiChung Chiun Liu
Feb 1, 2018·Analytical Chemistry·Subramaniam SomasundaramChristopher J Easley
Jul 21, 2017·ACS Sensors·Wendi ZhouShana O Kelley
Jul 2, 2019·Journal of the American Chemical Society·Subramaniam Somasundaram, Christopher J Easley
Feb 15, 2018·Journal of the American Chemical Society·Yanqin LvYuanjian Zhang

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