A highly sensitive and class-specific fluorescence polarisation assay for sulphonamides based on dihydropteroate synthase

Biosensors & Bioelectronics
Zhanhui WangJianzhong Shen

Abstract

We describe a fluorescence polarisation assay based on the use of dihydropteroate synthase (DHPS) and a fluorescence probe for multi-sulphonamide detection. Dihydropteridine pyrophosphate (DHPPP) was synthesised and acts as the first substrate for DHPS. Under optimised conditions, the half-maximal inhibitory concentrations (IC50) of the assay were less than 100ngmL(-1) for at least 29 sulphonamides and the time needed for the detection was less than 20min. More importantly, the assay revealed quite uniform affinities for all of the individual sulphonamides tested, which has never before been achieved in an antibody-based assay.

Citations

Jan 30, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chenglong LiZhanhui Wang
Dec 17, 2020·Sensors·Olga D HendricksonSergei A Eremin
Apr 17, 2021·Analytical Methods : Advancing Methods and Applications·Jinxin HeShaopeng Gu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Related Papers

The Medical Journal of Australia
K D FAIRLEY
Journal of the Indian Medical Association
B MUKERJI, G N SRIVASTAVA
The Practitioner
R G MOORE
© 2021 Meta ULC. All rights reserved